of an unknown protein are dissolved in enough solvent to make 5.00mL of solution. The osmotic pressure of this solution is measured to be 0.0815atm at 25.0°C.Calculate the molar mass of the protein. Round your answer to 3 significant digits.
Question
of an unknown protein are dissolved in enough solvent to make 5.00mL of solution. The osmotic pressure of this solution is measured to be 0.0815atm at 25.0°C.Calculate the molar mass of the protein. Round your answer to 3 significant digits.
Solution
To calculate the molar mass of the protein, we can use the formula for osmotic pressure:
Π = n/V * R * T
where: Π is the osmotic pressure, n is the number of moles of the solute, V is the volume of the solution, R is the ideal gas constant, and T is the temperature in Kelvin.
We know the osmotic pressure (Π = 0.0815 atm), the volume of the solution (V = 5.00 mL = 0.005 L), and the temperature (T = 25.0°C = 298.15 K). The ideal gas constant (R) is 0.0821 Latm/(Kmol).
We can rearrange the formula to solve for n (the number of moles of the solute):
n = Π * V / (R * T)
Substituting the known values:
n = 0.0815 atm * 0.005 L / (0.0821 Latm/(Kmol) * 298.15 K) = 0.0000167 mol
The molar mass of a substance is the mass of one mole of that substance. We know the mass of the protein (1.00 g) and we've calculated the number of moles (0.0000167 mol), so we can calculate the molar mass (M) as follows:
M = mass / n = 1.00 g / 0.0000167 mol = 59820.958 g/mol
Rounding to three significant digits, the molar mass of the protein is 59800 g/mol.
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