To precipitate proteins from solution, technicians often adjust the pH to the isoelectric point of the protein. The purpose in doing this is to:Question 18Select one:a.Promote repulsion by protein molecules having like charges.b.Increase hydration of acid and amine groups.c.Maintain disulfide linkagesd.Decrease solubility of protein molecules.
Question
To precipitate proteins from solution, technicians often adjust the pH to the isoelectric point of the protein. The purpose in doing this is to:Question 18Select one:a.Promote repulsion by protein molecules having like charges.b.Increase hydration of acid and amine groups.c.Maintain disulfide linkagesd.Decrease solubility of protein molecules.
Solution
The purpose of adjusting the pH to the isoelectric point of the protein is to decrease the solubility of protein molecules.
Here's why:
The isoelectric point (pI) of a protein is the pH at which the protein carries no net electrical charge. At this pH, any positive charges on the protein are balanced by an equal number of negative charges.
When a protein is in a solution at its isoelectric point, it is in its least soluble state. This is because at the pI, there is no net charge on the protein to interact with the water molecules and keep the protein in solution. As a result, the protein molecules aggregate and precipitate out of the solution.
So, the correct answer is d. Decrease solubility of protein molecules.
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