The tagging of a protein with the α-mating factor in yeast helps in the secretion of the recombinant protein. This is because the α-mating factor is a signal sequence that directs the protein to the secretory pathway. Here's a step-by-step explanation:

1. The gene of interest is fused with the gene for the α-mating factor in a plasmid. This creates a fusion protein that includes both the protein of interest and the α-mating factor.

2. This plasmid is then introduced into yeast cells.

3. Inside the yeast cell, the fusion protein is produced. The α-mating factor part of the protein acts as a signal sequence.

4. This signal sequence directs the protein to the endoplasmic reticulum, the first step in the secretory pathway.

5. The protein is then transported through the Golgi apparatus, where it may undergo further modifications.

6. Finally, the protein is secreted out of the cell.

So, the correct answer is (c) Secretion of the recombinant protein.

Question

The tagging of a protein with the α-mating factor in yeast helps in the secretion of the recombinant protein. This is because the α-mating factor is a signal sequence that directs the protein to the secretory pathway. Here's a step-by-step explanation:

1. The gene of interest is fused with the gene for the α-mating factor in a plasmid. This creates a fusion protein that includes both the protein of interest and the α-mating factor.

2. This plasmid is then introduced into yeast cells.

3. Inside the yeast cell, the fusion protein is produced. The α-mating factor part of the protein acts as a signal sequence.

4. This signal sequence directs the protein to the endoplasmic reticulum, the first step in the secretory pathway.

5. The protein is then transported through the Golgi apparatus, where it may undergo further modifications.

6. Finally, the protein is secreted out of the cell.

So, the correct answer is (c) Secretion of the recombinant protein.

Knowee AI · Accepted Answer

The tagging of a protein with the α-mating factor in yeast helps in the secretion of the recombinant protein. This is because the α-mating factor is a signal sequence that directs the protein to the secretory pathway. Here's a step-by-step explanation:

1. The gene of interest is fused with the gene for the α-mating factor in a plasmid. This creates a fusion protein that includes both the protein of interest and the α-mating factor.

2. This plasmid is then introduced into yeast cells.

3. Inside the yeast cell, the fusion protein is produced. The α-mating factor part of the protein acts as a signal sequence.

4. This signal sequence directs the protein to the endoplasmic reticulum, the first step in the secretory pathway.

5. The protein is then transported through the Golgi apparatus, where it may undergo further modifications.

6. Finally, the protein is secreted out of the cell.

So, the correct answer is (c) Secretion of the recombinant protein.

In yeast, the tagging of a protein with the -mating factor helps in (a) Tight regulation of protein expression (b) Cytoplasmic expression of the protein (c) Secretion of the recombinant protein (d) Proper folding of the overexpressed protein

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