Which of the following is a constitutive promotor in yeast expression system (a) GAPDH (b) AOX1 (c) MOX (d) AOX2
Question
Which of the following is a constitutive promotor in yeast expression system (a) GAPDH (b) AOX1 (c) MOX (d) AOX2
Solution
The constitutive promoter in a yeast expression system among the options provided is (a) GAPDH.
Here's a step-by-step explanation:
-
A constitutive promoter is a type of promoter that is continuously active, meaning it is always "on" and transcribing the downstream gene.
-
In the context of yeast expression systems, the GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) promoter is known to be constitutive. This means that the GAPDH promoter is always active and continuously drives the expression of the gene it controls.
-
On the other hand, AOX1 (Alcohol oxidase 1), MOX (Methanol oxidase), and AOX2 (Alcohol oxidase 2) are inducible promoters in yeast. They are not always active but can be turned "on" or "off" under certain conditions. For example, the AOX1 promoter is strongly induced by methanol.
So, the correct answer is (a) GAPDH.
Similar Questions
The inducible promoter MOX can be used in which of the following host expression system? (a) K. lactis (b) H. polymorpha (c) S. cerevisiae (d) S. occidentalis
Which of the following molecules matches its property?I Competitive inhibitor – similar shape to substrate and can occupy to the same active site. II Prosthetic group – decreases the rate of enzymatic reaction by changing its structure.III Active site – specific region of the enzymes which bind to the substrate*1 pointI and II onlyI and III onlyII and III onlyI, II and III
9 The trp operon is regulated by a(n)activator and inducerrepressor and inducerC) activator and co-repressord) repressor and co-repressor
Which of the following is the inducer of the lac operon in cells? Group of answer choiceslactose allolactosegalactoseglucoseisopropylthiogalactopyranoside
The enzymes in the replisome are active on Blank______.
Upgrade your grade with Knowee
Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.