f after running and analyzing the PCR products you found that all reactions including the positive control have no product or bands on the gel. What would be your conclusion? The human ACTB is unnecessary for the PCR The human ACTB sample used was too old The PCR was successful The PCR did not work The first step of the PCR should be avoided

What technique was used in the lab to analyze the products of the PCRs? Gel filtration chromatography SDS-PAGE Agarose gel electrophoresis Lipofection Transformation

The pCR4-TOPO plasmid used in the lab has 3956 bp and contains two EcoRI sites flanking the cloning site. The PCR product of the ACTB gene used for the ligation was ~2000 bp. After transforming and selecting the cells, a liquid LB media was grown form a colony overnight and the plasmid isolated from the cells. Thereafter the plasmid was digested with EcoRI and ran in an agarose gel. What approximate band sizes did you expect to see in the gel? 1 band of ~6000 bp 4 bands each of ~1500 bp 3 bands of ~1000 bp, ~2000 bp, and ~4000 bp 2 bands, one of ~2000 bp and one of ~4000 bp I expect to see no bands on the gel

What component of the PCR is omitted in the reaction used as Negative control? Template DNA dNTPs DNA polymerase Primers Reaction Buffer

Which of the locations would you expect to be present in this PCR product is a human gene? (There could be one answer or several. Give all answers that apply)

process of pcr