What component of the PCR is omitted in the reaction used as Negative control? Template DNA dNTPs DNA polymerase Primers Reaction Buffer
Question
What component of the PCR is omitted in the reaction used as Negative control? Template DNA dNTPs DNA polymerase Primers Reaction Buffer
Solution
The component of the PCR that is omitted in the reaction used as a Negative control is the Template DNA. This is because the purpose of a negative control is to check for contamination in the other reagents and to ensure that the amplification observed in the test samples is not due to any DNA present in the reagents. By omitting the template DNA, any amplification observed in the negative control can be attributed to contamination.
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