Plasmids used in cloning contain an antibiotic resistance gene.How does this help scientists?Choose 1 answer:Choose 1 answer:(Choice A) Bacteria without the plasmid will be easier for scientists to identify.ABacteria without the plasmid will be easier for scientists to identify.(Choice B) Only bacteria with the plasmid will grow on antibiotic containing nutrient plates. BOnly bacteria with the plasmid will grow on antibiotic containing nutrient plates.(Choice C) It prevents the bacteria from infecting the scientists during the cloning process.CIt prevents the bacteria from infecting the scientists during the cloning process.(Choice D) All of the prepared bacteria will be resistant to antibiotics.DAll of the prepared bacteria will be resistant to antibiotics.
Question
Plasmids used in cloning contain an antibiotic resistance gene.How does this help scientists?Choose 1 answer:Choose 1 answer:(Choice A) Bacteria without the plasmid will be easier for scientists to identify.ABacteria without the plasmid will be easier for scientists to identify.(Choice B) Only bacteria with the plasmid will grow on antibiotic containing nutrient plates. BOnly bacteria with the plasmid will grow on antibiotic containing nutrient plates.(Choice C) It prevents the bacteria from infecting the scientists during the cloning process.CIt prevents the bacteria from infecting the scientists during the cloning process.(Choice D) All of the prepared bacteria will be resistant to antibiotics.DAll of the prepared bacteria will be resistant to antibiotics.
Solution
The correct answer is (Choice B) Only bacteria with the plasmid will grow on antibiotic containing nutrient plates.
Here's why: When scientists are cloning genes, they often use bacteria as a host for the cloned gene. They insert the gene into a plasmid (a small, circular piece of DNA) and then introduce this plasmid into the bacteria. The plasmid also contains an antibiotic resistance gene.
After the bacteria have been exposed to the plasmid, they are grown on a nutrient plate that contains the specific antibiotic. Only the bacteria that have successfully taken up the plasmid (and therefore the antibiotic resistance gene) will be able to grow on this plate. This allows scientists to easily identify and isolate the bacteria that contain the cloned gene.
The other bacteria (those that did not take up the plasmid) will be killed by the antibiotic. So, the antibiotic resistance gene in the plasmid is a tool that scientists use to select for the bacteria that have been successfully transformed with the plasmid.
Similar Questions
Which of the following describes plasmids?Synthesise proteins.Controls what enters and exits the cell.Small circles of DNA that are used to share useful genes between bacteria.Contains all the instructions the bacterium needs to function and develop.
Discuss the role of plasmid in working of bacteria.
Bacterial pili can be related to the development of antibiotic resistance because Blank______.Multiple choice question.the pili themselves are involved in pumping antibiotics out of cellspili make the bacteria stronger overall, and therefore resistant to killingthe pili themselves are involved in preventing antibiotic entry into cellspili can be used to transfer genes that code for resistance mechanisms
Plasmids can be useful for many different elements of molecular microbiology but there are many considerations that dictate the features of a plasmid being used. If using a plasmid in order to express a large quantity of a particular protein (e.g. for protein purification) which of the following is NOT a requirement?The ability of the host bacterium to express GFP.A selectable marker that will facilitate retention of the plasmid.The presence of rep/ori genes that allows for replication of the plasmid in the bacterium of interest.A strong promoter that will drive transcription of the gene of interest and subsequent protein production.
6. A strain of bacteria living in a hospital is found to have a plasmid containing two genes: one forsex pilus construction, and one for tetracycline resistance. If these bacteria were to undergoconjugation with bacteria lacking this plasmid, and thereby transferred the plasmid to the otherbacteria, the most likely result would be:A. a genetically identical clone of bacteria containing the same plasmid.B. a possible spread of tetracycline resistance to other bacteria in the hospital.C. the subsequent loss of tetracycline resistance in the initially resistant strain.D. production of endospores in the bacterial progeny.E. temporary tetraploidy of the conjugating bacteria.
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