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udyGPTQuestion textIf DNA samples were successfully obtained from this case they could be used for DNA typing.Modern DNA fingerprinting techniques use STR analysis. 1. Explain what an STR is. (1 mark)2. How can STRs be used to create a DNA fingerprint? (3)3. Other than the fingerprint from the forensic "scene" what else is needed for a DNA fingerprint to be useful? (1 mark)

Question

udyGPTQuestion textIf DNA samples were successfully obtained from this case they could be used for DNA typing.Modern DNA fingerprinting techniques use STR analysis. 1. Explain what an STR is. (1 mark)2. How can STRs be used to create a DNA fingerprint? (3)3. Other than the fingerprint from the forensic "scene" what else is needed for a DNA fingerprint to be useful? (1 mark)

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Solution

  1. STR stands for Short Tandem Repeats. These are sequences of DNA that repeat themselves in a specific pattern. The number of repetitions varies among individuals, making them unique to each person.

  2. STRs can be used to create a DNA fingerprint through the following steps: a. DNA Extraction: The DNA is extracted from the cells and purified. b. DNA Amplification: The STR regions of the DNA are amplified using a technique called Polymerase Chain Reaction (PCR). This process creates multiple copies of the STR regions. c. Gel Electrophoresis: The amplified DNA is then separated by size using a process called gel electrophoresis. The smaller fragments move faster through the gel, creating a unique pattern of bands. d. Visualization: The pattern of bands is then visualized and can be compared to other DNA samples to identify matches.

  3. In addition to the fingerprint from the forensic "scene", a reference sample is also needed for a DNA fingerprint to be useful. This could be a DNA sample from a known individual or a database of DNA profiles for comparison. This allows for the identification of the individual from whom the DNA at the scene originated.

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