Below is a sequence of DNA that is going to be replicated. This is the sequence of the leading strand and only shows the position at which primase binds to synthesise RNA primers. Based on this sequence, which RNA primers would allow replication from both the leading and lagging strands?5’ ….CAAGGCTCCC….GCGCGTAGGA….GACCTTTATT 3’ Group of answer choicesFrom the leading strand: 5’ GGGAGCCUUG 3’ and 5’ GCGCGUAGGA 3’From the lagging strand: 5’ GACCUUUAUU 3’From the leading strand: 5’ GACCUUUAUU 3’From the lagging strand: 5’ GGGAGCCUUG 3’ and 5’ GCGCGUAGGA 3’From the leading strand: 5’ CAAGGCUCCC 3’ and 5’ GCGCGUAGGA 3’From the lagging strand: 5’ AAUAAAGGUC 3’From the leading strand: 5’ AAUAAAGGUC 3’From the lagging strand: 5’ CAAGGCUCCC 3’ and 5’ GCGCGUAGGA 3’
Question
Below is a sequence of DNA that is going to be replicated. This is the sequence of the leading strand and only shows the position at which primase binds to synthesise RNA primers. Based on this sequence, which RNA primers would allow replication from both the leading and lagging strands?5’ ….CAAGGCTCCC….GCGCGTAGGA….GACCTTTATT 3’ Group of answer choicesFrom the leading strand: 5’ GGGAGCCUUG 3’ and 5’ GCGCGUAGGA 3’From the lagging strand: 5’ GACCUUUAUU 3’From the leading strand: 5’ GACCUUUAUU 3’From the lagging strand: 5’ GGGAGCCUUG 3’ and 5’ GCGCGUAGGA 3’From the leading strand: 5’ CAAGGCUCCC 3’ and 5’ GCGCGUAGGA 3’From the lagging strand: 5’ AAUAAAGGUC 3’From the leading strand: 5’ AAUAAAGGUC 3’From the lagging strand: 5’ CAAGGCUCCC 3’ and 5’ GCGCGUAGGA 3’
Solution 1
The correct answer would be:
From the leading strand: 5’ GGGAGCCUUG 3’ and 5’ GCGCGUAGGA 3’ From the lagging strand: 5’ GACCUUUAUU 3’
Here's why:
DNA replication is semi-conservative and occurs in the 5' to 3' direction. The leading strand is synthesized continuously, while the lagging strand is synthesized in fragments (Okazaki fragments) due to the anti-parallel nature of DNA strands.
Primase synthesizes RNA primers complementary to the DNA strand. The RNA primer provides a 3' OH group for DNA polymerase to add nucleotides.
For the leading strand, the sequence 5’ ….CAAGGCTCCC….GCGCGTAGGA….GACCTTTATT 3’ would have the RNA primers 5’ GGGAGCCUUG 3’ and 5’ GCGCGUAGGA 3’.
For the lagging strand, the sequence would be read in the opposite direction, and the RNA primer would be 5’ GACCUUUAUU 3’.
Solution 2
The correct answer is:
From the leading strand: 5’ GGGAGCCUUG 3’ and 5’ GCGCGUAGGA 3’
From the lagging strand: 5’ GACCUUUAUU 3’
Here's why:
DNA replication is semi-conservative and occurs in the 5' to 3' direction. The leading strand is synthesized continuously, while the lagging strand is synthesized in fragments (Okazaki fragments) due to the anti-parallel nature of DNA strands.
Primase synthesizes RNA primers complementary to the DNA strand. The RNA primer provides a 3' OH group for DNA polymerase to add nucleotides.
For the leading strand, the sequence 5’ ….CAAGGCTCCC….GCGCGTAGGA….GACCTTTATT 3’ would have the primers 5’ GGGAGCCUUG 3’ and 5’ GCGCGUAGGA 3’ because these are complementary to the DNA sequence.
For the lagging strand, the primer would be 5’ GACCUUUAUU 3’ because it is complementary to the DNA sequence. The lagging strand is synthesized in the opposite direction (3' to 5') in small fragments, so the primer is at the end of the sequence.
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