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Consider a given stretch of mRNA sequence. Which of the following shall you consider singe stranded DNA probe for the given mRNA sequence? 3' TGAACCTGAGTA 5' 5' TGAACCTGAGTA 3' 3' UGAACCUGAGUA 5' 5' UGAACCUGAGUA 3'

Question

Consider a given stretch of mRNA sequence. Which of the following shall you consider singe stranded DNA probe for the given mRNA sequence? 3' TGAACCTGAGTA 5' 5' TGAACCTGAGTA 3' 3' UGAACCUGAGUA 5' 5' UGAACCUGAGUA 3'

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Solution

The single-stranded DNA probe for the given mRNA sequence would be 5' ACTTGGACTCAT 3'.

Here's the step-by-step process:

  1. The given mRNA sequence is 3' UGAACCUGAGUA 5'.

  2. DNA is complementary to RNA, so to find the DNA sequence, you would replace all the U's in the RNA sequence with T's (since in DNA, A pairs with T and C pairs with G).

  3. So, the DNA sequence complementary to the given mRNA sequence would be 3' TGAACCTGAGTA 5'.

  4. However, a probe is usually designed to be the reverse complement of the target sequence. This is because DNA is antiparallel, meaning the two strands run in opposite directions.

  5. Therefore, to get the probe sequence, you would reverse the sequence and replace A with T, T with A, C with G, and G with C.

  6. So, the single-stranded DNA probe for the given mRNA sequence would be 5' ACTTGGACTCAT 3'.

This problem has been solved

Similar Questions

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The complementary messenger RNA strand that would be synthesized from the DNA template sequence of 5' CTGAC 3' would be _________blank.Multiple Choice3' AGTUG 5'5' GACGU 3'3' GACTG 5'5' UGACU 3'3' GACUG 5'

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