Researchers are studying the last two phases of mitosis, anaphase and telophase, in actively dividing cancer cells. Different fluorescent probes are used to label various cellular components in these cells. Under the microscope, fluorescently labeled DNA appears blue, the nuclear envelope appears green, and the mitotic spindle appears red. Given this, green fluorescence would be most intense during:A.anaphase only.B.telophase only.C.both anaphase and telophase.D.neither anaphase nor telophase.
Question
Researchers are studying the last two phases of mitosis, anaphase and telophase, in actively dividing cancer cells. Different fluorescent probes are used to label various cellular components in these cells. Under the microscope, fluorescently labeled DNA appears blue, the nuclear envelope appears green, and the mitotic spindle appears red. Given this, green fluorescence would be most intense during:A.anaphase only.B.telophase only.C.both anaphase and telophase.D.neither anaphase nor telophase.
Solution
The answer is B. telophase only.
Here's the step-by-step reasoning:
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The nuclear envelope, which appears green when fluorescently labeled, disassembles in prophase (the first phase of mitosis) and does not reassemble until telophase (the last phase of mitosis).
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During anaphase, the third phase of mitosis, the nuclear envelope is still disassembled, so there would be little to no green fluorescence.
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During telophase, the nuclear envelope reassembles around the two sets of daughter chromosomes, so the green fluorescence would be most intense during this phase.
Therefore, green fluorescence, which indicates the presence of the nuclear envelope, would be most intense during telophase only.
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