What DNA tool can help produce substances vital to the organisms’ body like insulin by inserting genes from humans to the genetic ring of the bacteria?Group of answer choicesDNA fingerprintingDNA sequencingRecombinant DNAPolymerase Chain Reaction (PCR)
Question
What DNA tool can help produce substances vital to the organisms’ body like insulin by inserting genes from humans to the genetic ring of the bacteria?Group of answer choicesDNA fingerprintingDNA sequencingRecombinant DNAPolymerase Chain Reaction (PCR)
Solution
The DNA tool that can help produce substances vital to the organisms’ body like insulin by inserting genes from humans to the genetic ring of the bacteria is Recombinant DNA.
Here's a step-by-step explanation:
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Isolation of the human insulin gene: The first step in this process is to isolate the human gene that codes for insulin. This is done using restriction enzymes, which are proteins that can cut DNA at specific sequences.
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Insertion of the insulin gene into a plasmid: Once the insulin gene has been isolated, it is inserted into a plasmid, which is a small, circular piece of DNA found in bacteria. This is done using the same restriction enzymes, which create complementary "sticky ends" on the insulin gene and the plasmid, allowing them to be joined together.
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Transformation of bacteria: The plasmid containing the insulin gene is then introduced into bacteria. This process is known as transformation. The bacteria incorporate the plasmid into their own DNA, effectively becoming factories for the production of human insulin.
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Production
Similar Questions
A bacterium that has received recombinant DNA containing the gene for human insulin willMultiple choice question.no longer produce human insulin but will instead produce only bacterial insulin.possess the gene but will not produce the protein product of the gene.replicate the gene along with the rest of their DNA but only produce protein product of the bacterial genes.produce human insulin along with the other proteins it normally produces.
The technique used to insert the human insulin gene into bacterial cells is called: (1 Point)Critical Thinking Questiona) Genetic engineeringb) Cloningc) Recombinationd) Hybridization
Identify the tools used to perform DNA extraction and PCR
What is used to chop DNA into fragments?Group of answer choicesRestriction EnzymesDNA scissorsVery tiny chef knivesScissor Enzymes
After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to:Group of answer choicesverify that the desired DNA sequence has been amplifiedamplify the DNAconvert cDNA into genomic DNAconvert cDNA into messenger RNAsynthesize primer DNA molecules
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