After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to:Group of answer choicesverify that the desired DNA sequence has been amplifiedamplify the DNAconvert cDNA into genomic DNAconvert cDNA into messenger RNAsynthesize primer DNA molecules
Question
After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to:Group of answer choicesverify that the desired DNA sequence has been amplifiedamplify the DNAconvert cDNA into genomic DNAconvert cDNA into messenger RNAsynthesize primer DNA molecules
Solution
After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to verify that the desired DNA sequence has been amplified.
Here are the steps:
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After PCR, the amplified DNA product is loaded into the wells of an agarose gel. Agarose gel is a porous matrix through which molecules can travel when an electric field is applied.
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The gel is placed in a buffer solution, and an electric current is applied. DNA is negatively charged, so it will move towards the positive electrode.
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Smaller DNA fragments move through the gel more quickly than larger ones. This size-based separation allows us to distinguish between DNA fragments of different lengths.
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After the electrophoresis is complete, the gel is stained with a dye that binds to DNA. This makes the DNA visible under UV light.
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The presence of bands in specific locations on the gel indicates that DNA fragments of a particular size are present. If the PCR was designed to amplify a specific DNA sequence, the presence of a band of the expected size indicates that the desired sequence has been amplified.
Similar Questions
The polymerase chain reaction is a method of A) performing gel electrophoresis. B) DNA amplification. C) making cDNA. D) making an RNA probe
Which of these enzymes is used in a PCR? RNA polymerase EcoRI DNA polymerase DNA ligase DNA reverse transcriptase
1. QuestionPolymerase chain reaction (PCR) is a DNA manipulation technique used to: Cut DNA molecules at specific sequences of nucleotides Separate DNA molecules according to size Join molecules of DNA together Copy specific regions of DNA2. QuestionWhat occurs in the first step of PCR? DNA strands are separated by heating them Primers bind to target DNA strands Taq DNA polymerase builds a new DNA molecule using the target strand as a template3. QuestionWhat occurs in the second step of PCR? DNA strands are separated by heating them Primers bind to target DNA strands Taq DNA polymerase builds a new DNA molecule using the target strand as a template4. QuestionWhat occurs in the final step of PCR? DNA strands are separated by heating them Primers bind to target DNA strands Taq DNA polymerase builds a new DNA molecule using the target strand as a template5. QuestionWhy are the two strands of nucleotides in a DNA molcule required to be separated during PCR?6. QuestionReferring to the image below, which primer will bind to the sequence of nucleotides? Primer 1 Primer 2 Neither primer will bind7. QuestionHow does Taq DNA polymerase extend a new strand of nucleotides? By adding nucleotides that are identical to the original strand By adding nucleotides that are complementary to the original strand By joining primers together
Identify the tools used to perform DNA extraction and PCR
PCR is a technique used to amplify trace amounts of ______ to be used in scientific and forensic analyses.Multiple choice question.stem cellsDNAproteinRNA
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