transgenic mice

transgenic animal

A scientist wishes to generate transgenic mice, which express a particular exogenous gene only in their muscles. It is expected that the mice will transmit the exogenous gene to their offspring. Which of the following approaches to generate the mice would be most effective?  The gene could be injected directly into the muscle of adult mice. mRNA from the gene, made by transcription in vitro could be injected into muscle of an adult mouse. CRISPR could be used to disrupt a muscle-specific myosin gene, followed by repair by non-homologous recombination. The gene could be fused to the promoter from a muscle-specific myosin gene and injected into newly fertilised mouse eggs. Newly fertilised eggs could be infected with Agrobacterium tumefaciens cells that carry the gene on the Ti plasmid.

A- producing large numbers of genetically identical model Transgenic mice that show symptoms of diabetes, B-growing a replacement kid me identically tissue-matched to an individual patient, C-training replacement hearts from transgenic pigs, partially tissue matched to humans, D-genetically manipulating cells of one adult to cure a genetic disease in the individual, E-altering a prokaryotic pathogen for use as a vaccine. Match the correct letters to the names. No letter should be used more than once: xenotransplation, somatic gene therapy, non-reproductive cloning, animal reproductive cloning

Mouse DNA has mutated about ____________as fast as humans DNA.

Which two applications of gene cloning involve transgenic organisms?