2. A student group is attempting to construct a restriction map for plasmid p1002B using tworestriction enzymes EcoR1 and BamHI. After carrying out the digestion reactions andelectrophoresis separation through an agarose gel, the image below was obtained.Which of the following sketches best represents the restriction map derived from the gel data?Answer is A.

Consider the given following plasmid map carefully. Rohilla performed digestion experiment of the plasmid and got the following band pattern after Agarose Gel Electrophoresis. Lane A has one band of 29 kb and Lane B has three bands of 6 kb,11 kb and 12 kb. What does the Lane A and Lane B correspond to? Lane A- Double digestion by EcoR1 followed by Stu1 Lane B – Digestion by Stu1 Lane A – Digestion by Stu1 Lane B – Double digestion by Stu1 followed by EcoR1 Lane A - Double digestion by EcoR1 followed by BamH1 Lane B - Double digestion by EcoR1 followed by Stu1 Lane A - Double digestion by EcoR1 followed by Stu1 Lane B - Double digestion by EcoR1 followed by BamH1

A circular plasmid has three different but unique restriction sites for enzymes‘a’, ‘b’ and ‘c.’ When enzymes ‘a’ and ‘b’ are used together, two fragments ofequal size are generated. Enzyme ‘c’ creates fragments of equal size only fromone of the fragments generated by those cleaved by ‘a’ and ‘b’. The plasmid istreated with a mixture of ‘a’, ‘b’ and ‘c’ and analysed by agarose gelelectrophoresis. The number of bands observed in the gel is

In genetic engineering, restriction enzymes cleave the DNA at a specific site known as _____a) restrictionb) recognitionc) promoterd) sense

ich of the below is the most accurate description of restriction digestion, as used in a cloning procedure?Group of answer choicesCutting RNA at random locationsCutting plasmids into equally-sized piecesCutting specific locations in enzymes to inactivate themCutting DNA at defined locationsCutting promoter sequences out of an mRNA strand

The recognition sequences of four Type-II restriction enzymes (RE) are givenbelow. The symbol (↓) indicates the cleavage site. Identify the RE that generatessticky ends.(A) RE1 - 5̍ G↓GATCC 3̍(B) RE2 - 5̍ CTG↓CAG 3̍(C) RE3 - 5̍ CCC↓GGG 3̍(D) RE4 - 5̍ AG↓CT 3̍