Which reaction in DNA replication is catalysed by DNA ligase?Group of answer choicesFormation of a phosphodiester linkage between two Okazaki fragmentsProof reading the template strand for errorsBase pairing of the template to the newly synthesised strandAddition of nucleotides to the lagging strand
Question
Which reaction in DNA replication is catalysed by DNA ligase?Group of answer choicesFormation of a phosphodiester linkage between two Okazaki fragmentsProof reading the template strand for errorsBase pairing of the template to the newly synthesised strandAddition of nucleotides to the lagging strand
Solution
The reaction in DNA replication that is catalysed by DNA ligase is the formation of a phosphodiester linkage between two Okazaki fragments.
Here are the steps:
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During DNA replication, the DNA molecule unzips, and new nucleotides are added to each strand by DNA polymerase.
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On the leading strand, this process is continuous. However, on the lagging strand, replication occurs in segments known as Okazaki fragments.
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These fragments are separate pieces of DNA, and they need to be joined together to form a continuous strand.
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This is where DNA ligase comes in. DNA ligase catalyses the formation of a phosphodiester bond between the 3' end of one fragment and the 5' end of the next.
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This reaction joins the Okazaki fragments together, resulting in a continuous DNA strand.
So, DNA ligase plays a crucial role in DNA replication, ensuring that the newly synthesized DNA is continuous and complete.
Similar Questions
During DNA replication:Ligase replaces RNA primers with DNA nucleotides.DNA polymerase III pairs Guanine with Cytosine.DNA polymerase I pairs Adenine with Cytosine.Okazaki fragments are created on the leading strand.
Which is true during DNA replication?Group of answer choicesDNA ligase is the last step of the polymerisation reaction for the lagging strand, but is not used on the leading strand.DnaA binds to regions that are rich in AT as this purine-purine base pairing forms only two hydrogen bonds.The β sliding clamp prevents the dissociation of DNA polymerase III, increasing the length of DNA each polymerase can synthesise.DNA gyrase binds to and applies tension to the lagging strand.
Which type of enzyme catalyses the synthesis of DNA?Group of answer choicesDNA polymerasePrimaseHelicaseDNA ligase PreviousNext
n DNA replication, the Okazaki fragments are joined by(A) DNA helicase(B) DNA ligase(C) DNA polymerase(D) DNA primase
Many segments of these are joined together to make up the lagging strand of newly synthesized DNA.Group of answer choicesprimerOkazaki fragmenttelomereorigin of replicationNext
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