You have been tasked with developing a new polymerase chain reaction (PCR) protocol for detecting the DNA virus Adenovirus type 5 in patient samples. Which of the following is unnecessary to prepare for this PCR?Group of answer choicesDeoxynucleoside triphosphates (dNTPs)A DNA polymeraseThe sequence of the virus genomeConversion of the genome to cDNA using a reverse transcriptase

Which of these enzymes is used in a PCR? RNA polymerase EcoRI DNA polymerase DNA ligase DNA reverse transcriptase

Which of the following enzymes is used by RNA viruses to synthesise DNA using an RNA template?Group of answer choicesRNA polymerase IReverse transcriptaseRestriction endonucleaseDNA polymerase I

Organize the following steps of a PCR cycle in the correct sequence.The mixture is cooled to allow the binding of primers to the end of target DNA sequences.Free nucleotides are added in the 5’ to 3’ direction by the Taq DNA polymerase.The reaction mixture is heated to separate the double-stranded DNA.Group of answer choices1, 2, 31, 3, 22, 1, 32, 3, 13, 1, 23, 2, 1

Which of the following correctly matches the step of polymerase chain reaction (PCR) with its events?Choose 1 answer:Choose 1 answer:(Choice A)   Extension - reaction is cooled and primers bind to complementary sequences on template DNA.AExtension - reaction is cooled and primers bind to complementary sequences on template DNA.(Choice B)   Annealing - Taq polymerase extends the primers, synthesizing DNABAnnealing - Taq polymerase extends the primers, synthesizing DNA(Choice C, Checked)   Denaturation - high temperature separates the DNACDenaturation - high temperature separates the DNA

Which of the following statements is true about diagnostic testing for viruses Group of answer choicesSerology can only be used to determine exposure to a virus, not immunityPCR is commonly used to determine if a patient is infectiousWhole genome sequencing is useful for evaluating viral transmissionSample collection does not influence PCR assay performanceNone of the above are true