Which of the following statements best describes the amplification of DNA in each PCR cycle?Group of answer choicesThe number of double stranded DNA molecules produced that contain the target sequence DOUBLES with each PCR cycleThe number of double stranded DNA molecules produced that contain the target sequence INCREASES BY 2 molecules with each PCR cycleThe number of double stranded DNA molecules produced that contain the target sequence INCREASES BY 1 molecule with each PCR cycleThe number of double stranded DNA molecules produced that contain the target sequence REMAINS CONSTANT with each PCR cycle
Question
Which of the following statements best describes the amplification of DNA in each PCR cycle?Group of answer choicesThe number of double stranded DNA molecules produced that contain the target sequence DOUBLES with each PCR cycleThe number of double stranded DNA molecules produced that contain the target sequence INCREASES BY 2 molecules with each PCR cycleThe number of double stranded DNA molecules produced that contain the target sequence INCREASES BY 1 molecule with each PCR cycleThe number of double stranded DNA molecules produced that contain the target sequence REMAINS CONSTANT with each PCR cycle
Solution
The statement that best describes the amplification of DNA in each PCR cycle is: "The number of double stranded DNA molecules produced that contain the target sequence DOUBLES with each PCR cycle".
Here's why:
PCR (Polymerase Chain Reaction) is a method widely used in molecular biology to make several copies of a specific DNA segment. During PCR, the DNA sample is heated to separate its two complementary strands. Once the strands are separated, short pieces of DNA known as primers are added. These primers are designed to bind to a specific location on the DNA.
Then, an enzyme called DNA polymerase is added, which starts to make a new DNA strand by adding nucleotides, the building blocks of DNA, to the primer. This process is repeated many times in a cycle of heating and cooling, with each cycle doubling the amount of DNA.
So, in the first cycle, you have 2 copies. In the second cycle, those 2 copies each get replicated, so you have 4. In the third cycle, those 4 each get replicated, so you have 8, and so on. This is why the number of double stranded DNA molecules that contain the target sequence doubles with each PCR cycle.
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