High-performance liquid chromatography (HPLC) uses a polar stationary phase together with a non-polar mobile phase.Which of the following molecules will have the least retention time (Rt) on HPLC? a.Molecule (II)b.Molecule (IV)c.Molecule (III)d.Molecule (I)

Which one of the below statements is wrong regarding the HPLC technique? Choose one answer. Retention time for each component can be obtained High temperature is being utilized The mobile phase is a liquid Is one of the chromatography techniques

In reversed phase chromatography, a non-polar stationary phase is used, non-polar analytes are retained more strongly, and a more non-polar eluent will cause shorter retention times for analytes in a chromatographic analysis.Group of answer choicesTrueFalse

In reverse phase chromatography, the stationary phase is made

The correct statement among the following, for a "chromatography" purification method is :JEE Main 2024 (06 Apr Shift 2)AOrganic compounds run faster than solvent in the thin layer chromatographic plate.BRfR𝑓 is an integral value.CRfR𝑓 of a polar compound is smaller than that of a non-polar compound.DNon-polar compounds are retained at top and polar compounds come down in column chromato

2. A reversed-phase HPLC column of dimensions 4.6 mm diameter by 150 mm length is used at a liquid flow rate of 1.0 mL/min. An unretained compound elutes after 0.82 min. A compound with a KOW of 25 takes 2.88 min. to elute when using a mobile phase of 90% water/10% acetonitrile. Assuming the KC is equal to the KOW, calculate the stationary phase volume. A. 0.049 mL B. 0.082 mL C. 0.50 mL D. 1.00 mL E. 1.12 mL