The most prevalent poor metabolizers (PM) variant genotypes of CYP2C19 are  Blank 1 Question 2 and  Blank 2 Question 2 in Asian

Genotypes of one pair of the following genes could benefit patients treated with warfarin.Question 9Answera.CYP2C9 and CYP2B1b.CYP2C19*2 and CYP2C9*3c.HLA-B∗1502 and CYP2D6d.CYP2C9 and VKORC1

Two metabolites of a new compound were identified after incubation with pooled human liver microsomes, a desmethyl metabolite and a ring hydroxylated metabolite. The new compound was incubated with samples from a human liver microsome bank, and the amount of metabolite formed was compared with activities for probe substrates. The data are available in the table on the next slide. Correlation with CYP probe substrates Metabolite CYP2A6 CYP2B6 CYP2C19 CYP2D6 CYP3A4 Desmethyl 0.22 NS 0.33 NS 0.87 *** 0.41 NS 0.52 * R-hydroxyl 0.41 * 0.79 *** 0.24 * 0.21 NS 0.44 * Data are correlation coefficients with associated P values (a) The metabolites were identified and quantified using liquid chromatography, coupled to mass spectrometry (triple quadrupole) using selected reaction monitoring. Give a brief explanation of selected reaction monitoring, including a description of how each of the quadrupole mass analysers contributes to detection. (b) What would be the appropriate units for metabolite formation and probe substrate activity for these correlation studies? (c) A significant P value (shown by the asterisks) shows that the gradient of the regression line is significantly different from what value? (d) What do the correlation data suggest about the enzyme responsible for metabolite formation? (e) What further studies would you carry out to gain further information and confirmationTwo metabolites of a new compound were identified after incubation with pooled human liver microsomes, a desmethyl metabolite and a ring hydroxylated metabolite. The new compound was incubated with samples from a human liver microsome bank, and the amount of metabolite formed was compared with activities for probe substrates. The data are available in the table on the next slide. Correlation with CYP probe substrates Metabolite CYP2A6 CYP2B6 CYP2C19 CYP2D6 CYP3A4 Desmethyl 0.22 NS 0.33 NS 0.87 *** 0.41 NS 0.52 * R-hydroxyl 0.41 * 0.79 *** 0.24 * 0.21 NS 0.44 * Data are correlation coefficients with associated P values (a) The metabolites were identified and quantified using liquid chromatography, coupled to mass spectrometry (triple quadrupole) using selected reaction monitoring. Give a brief explanation of selected reaction monitoring, including a description of how each of the quadrupole mass analysers contributes to detection. (b) What would be the appropriate units for metabolite formation and probe substrate activity for these correlation studies? (c) A significant P value (shown by the asterisks) shows that the gradient of the regression line is significantly different from what value? (d) What do the correlation data suggest about the enzyme responsible for metabolite formation? (e) What further studies would you carry out to gain further information and confirmation

Two metabolites of a new compound were identified after incubation with pooled human liver microsomes, a desmethyl metabolite and a ring hydroxylated metabolite. The new compound was incubated with samples from a human liver microsome bank, and the amount of metabolite formed was compared with activities for probe substrates. The data are available in the table on the next slide. Correlation with CYP probe substrates Metabolite CYP2A6 CYP2B6 CYP2C19 CYP2D6 CYP3A4 Desmethyl 0.22 NS 0.33 NS 0.87 *** 0.41 NS 0.52 * R-hydroxyl 0.41 * 0.79 *** 0.24 * 0.21 NS 0.44 * Data are correlation coefficients with associated P values (a) The metabolites were identified and quantified using liquid chromatography, coupled to mass spectrometry (triple quadrupole) using selected reaction monitoring. Give a brief explanation of selected reaction monitoring, including a description of how each of the quadrupole mass analysers contributes to detection. (b) What would be the appropriate units for metabolite formation and probe substrate activity for these correlation studies? (c) A significant P value (shown by the asterisks) shows that the gradient of the regression line is significantly different from what value? (d) What do the correlation data suggest about the enzyme responsible for metabolite formation? (e) What further studies would you carry out to gain further information and confirmation

YouWhat is the genotype of individual II-5?

A person’s genotype is their genetic makeup and includes: