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Amongst the following, who was the first to isolate DNA, from the nucleus of the pus cells?J.D. WatsonFriedrich MiescherHofmeisterStrasburger

Question

Amongst the following, who was the first to isolate DNA, from the nucleus of the pus cells?J.D. WatsonFriedrich MiescherHofmeisterStrasburger

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Solution

The first person to isolate DNA from the nucleus of pus cells was Friedrich Miescher. Here are the steps he took:

  1. Miescher began his work by collecting pus from discarded surgical bandages. The pus contained a high number of white blood cells, which are rich in nuclei.

  2. He then treated the cells with a solution of sodium sulfate and sodium chloride to break down the proteins and lipids in the cell membranes, leaving only the nuclei.

  3. Miescher further treated the nuclei with an alkaline solution and then with acid, which caused the nuclei to break down and release their contents.

  4. Among the substances released was a substance Miescher called "nuclein" because it came from the nuclei. This substance was high in phosphorus and resistant to protein-digesting enzymes, which led Miescher to believe it was a new type of molecule.

  5. Later research confirmed that "nuclein" was indeed DNA. Thus, Friedrich Miescher was the first person to isolate DNA, although he did not fully understand what he had discovered at the time.

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Similar Questions

Which among the following was done by Johannes Purkinje?Invented the first compound microscopeFirst observed living cells of bacteria, sperm and protozoaNamed the fluid content of the cells as protoplasmObservation of ribosomes in animal cell

Who discovered the nucleus for the first time?c. Why is it called the ‘ control center’ of the cell ?

Name the person who discovered the nucleus.

The first step of the lab project was the extraction and purification of genomic DNA. What technique was used to isolate DNA molecules from the other type of molecules present in the cell? Ultra-Centrifugation Ion-Exchange chromatography Microscopy Gel Electrophoresis Cloning

Who described chromosome structure that appeared in nucleus during cell division?FlemmingStrasburgerWaldeyerSchwann

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