Give a step-by-step procedure for preparing a wet mount slide to view onion cells under a light microscope.
Question
Give a step-by-step procedure for preparing a wet mount slide to view onion cells under a light microscope.
Solution
Sure, here is a step-by-step procedure for preparing a wet mount slide to view onion cells under a light microscope:
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Gather your materials: You will need an onion, a knife, a microscope slide, a cover slip, a dropper, iodine solution, and a light microscope.
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Cut a small piece of onion using the knife. Be careful not to cut yourself.
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Peel off a thin layer of the onion. This will be your sample.
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Place the onion layer on the microscope slide. Try to lay it flat to prevent it from folding or rolling up.
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Add a drop or two of iodine solution to the onion layer. The iodine will help to stain the cells, making them easier to see under the microscope.
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Carefully place the cover slip over the onion layer. To do this, hold the cover slip at an angle and slowly lower it onto the slide. This will help to prevent air bubbles from getting trapped under the cover slip.
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Check the slide for air bubbles. If you see any, gently tap the cover slip to try to remove them.
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Place the slide on the microscope stage and secure it with the stage clips.
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Start with the lowest magnification on your microscope and focus on the onion cells. Once you have them in focus, you can increase the magnification for a closer look.
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Observe the onion cells through the microscope. You should be able to see the cell walls, the nucleus, and possibly some other cell structures.
Remember to handle all materials with care and clean up your workspace when you're done.
Similar Questions
A student prepared some onion cells. The student viewed the onion cells using a light microscope. This is the method used. 1. Cut an onion into pieces using a sharp knife. 2. Peel off a thin layer of onion epidermis from one piece of onion. 3. Place the onion epidermis onto a microscope slide in a single flat layer. 4. Add three drops of iodine solution. 5. Slowly lower a cover slip at an angle onto the onion epidermis. 6. Place the slide on the stage of the microscope. 4. Page 6 of 16Sugars absorbed via active transport would show a decrease in aborption rate where there is no respiration occuring as it needs energy to carry out active transport and from the table we can see that the sugars glucose and galactose both significantly decreased whichi informs us that those two sugards can be absorbed by active transportBecause every single sugar got absorbed which informs us that some form of diffusionhas taken place(a) The table below shows a risk assessment for this experiment. Complete the table.HazardRiskPlan to minimise risk Iodine solution is an irritant May cause allergic reaction or skin rashSharp knife(2) (b) Give a reason for each of the following steps in the method. A thin layer of onion epidermis is used. ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ Iodine solution is added to the onion epidermis. ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ The cover slip is lowered onto the onion epidermis at an angle. ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ (3) Page 7 of 16Figure 1 shows what the student saw under the microscope at a magnification of ×400. Figure 1 (c) The length of cell Z in Figure 1 is 4.8 cm. Calculate the real length of cell Z. Give your answer in micrometres (μm). ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ Real length of cell Z = _______________μm (5) Page 8 of 16Figure 2 shows the student’s drawing of Figure 1. Figure 2 (d) Give two ways the student could improve the drawing in Figure 2. 1 _________________________________________________________________ ___________________________________________________________________ 2 _________________________________________________________________ ___________________________________________________________________ (2) (e) Onion cells can be seen using an electron microscope. Give two ways onion cells would look different when seen using an electron microscope. 1 _________________________________________________________________ ___________________________________________________________________ 2 _________________________________________________________________ ___________________________________________________________________ (2) (Total 14 marks)
viewing an onion cell through the microscopeWrite up a mini report for the experimentHeading/titlethe purposeequipmentrisk assessmentmethodWrite up a risk assessment for the practical
Homework:Watch videos and prepare for the practical on Monday.Practical - making a wet mount slide and and viewing an onion cell through the microscopeWrite up a mini report for the experimentHeading/titlethe purposeequipmentrisk assessmentmethodWrite up a risk assessment for the practical
Sasha is using the microscope below to look at onion cells. Once she has the onion cells mostly in focus, what does she use to make the image even clearer?
Recall what the part of the microscope that controls how much light passes up towards the slide is called.
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