Which of the following statements is correct regarding agarose gel electrophoresis? Question 3Answer a. A small DNA fragment will travel faster than a large fragment in a direction based on the fragment's negative charge. b. A small DNA fragment will travel faster than a large fragment in a direction based on the fragment's positive charge. c. A small DNA fragment will travel slower than a large fragment in a direction based on the fragment's positive charge. d. A small DNA fragment will travel slower than a large fragment in a direction based on the fragment's negative charge.
Question
Which of the following statements is correct regarding agarose gel electrophoresis?
Question 3Answer
a. A small DNA fragment will travel faster than a large fragment in a direction based on the fragment's negative charge.
b. A small DNA fragment will travel faster than a large fragment in a direction based on the fragment's positive charge.
c. A small DNA fragment will travel slower than a large fragment in a direction based on the fragment's positive charge.
d. A small DNA fragment will travel slower than a large fragment in a direction based on the fragment's negative charge.
Solution
The correct statement regarding agarose gel electrophoresis is:
a. A small DNA fragment will travel faster than a large fragment in a direction based on the fragment's negative charge.
This is because DNA fragments are negatively charged due to their phosphate backbone. In agarose gel electrophoresis, an electric field is applied that pulls the negatively charged DNA fragments towards the positive electrode. Smaller fragments move through the gel more quickly than larger ones, so they travel further in a given amount of time.
Similar Questions
DNA fragments separate on an electrophoresis gel with the A) largest segments traveling furthest. B) smallest segments traveling furthest. C) most negatively charged pieces traveling quickest. D) most positively charged pieces moving backward
Gel electrophoresis separates fragments of DNA according to what property? Length in nucleotides Organism they originate from Protein they code for2. QuestionWhat is the overall charge on DNA? Positive charge Negative charge DNA has no charge3. QuestionReferring to the image below, on which side of the gel tank should the positive electrode be placed so that DNA in the well migrates in the direction of the arrow? Left side Right side4. QuestionTwo fragments of DNA are loaded into a well on a gel. One fragment is 50 nucleotides in length, the other fragment is 200 nucleotides in length. A current is applied to the gel for 10 minutes. Which fragment will migrate further through the gel? The fragment that is 50 nucleotides in length The fragment that is 200 nucleotides in length Both fragments will travel the same distance5. QuestionWhat is the purpose of using a stain that binds to DNA and fluoresces under UV light? It assists with loading DNA fragments into wells It gives the DNA a negative charge so it will travel through the gel It gives the DNA a positive charge so it will travel through the gel It allows us to visualise where DNA fragments are positioned along the gel6. QuestionThe image below shows the results of a gel run. Based on these results, which of the following statements is correct? The DNA fragments in band A are shorter than the DNA fragments in band C The DNA fragments in band A are longer than the DNA fragments in band C The DNA fragments in band A are roughly the same length as the DNA fragments in band C7. QuestionBased on the gel results in the previous question, which of the following statements is correct? The DNA fragments in band B are shorter than the DNA fragments in band C The DNA fragments in band B are longer than the DNA fragments in band C The DNA fragments in band B are roughly the same length as the DNA fragments in band C8. QuestionEach person inherits two copies of a certain gene from their biological parents. There are two versions of the gene that can be inherited, version A and version B. Version A is longer than version B.DNA samples are collected from three patients. The gene of interest is copied using PCR and each sample is loaded into wells on a gel. The gel is run and a DNA profile is obtained (as seen in the image below). Which versions of the gene does each patient have? Justify your response.
Consider the following gel electrophoresis for a single protein from 7 distinct individuals. Based on these results, which of the following statements is CORRECT?The protein displays polymorphism but not heterozygosityThe protein displays heterozygosity but not polymorphismThe protein displays heterozygosity and polymorphismYou can distinguish all of the DNA sequence differences among these 7 individuals in the genes encoding this protein from this gel electrophoresi
Based on the gel results in the previous question, which of the following statements is correct? The DNA fragments in band B are shorter than the DNA fragments in band C The DNA fragments in band B are longer than the DNA fragments in band C The DNA fragments in band B are roughly the same length as the DNA fragments in band C
The results of gel electrophoresis are shown below, with four different strands of DNA labeled.Which strand of DNA is the shortest?A.2B.4C.3D.1
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