Significance of Protein - Drug Binding|

The plasma protein binding of the drug is characterized by:A. The protein-bound drugs are distributed and metabolizedB. The unbound fraction of the drug is activeC. Bound form is eliminated by the kidney more than free formD. Increased binding to plasma protein will increase drug distribution

You have identified a novel drug that can bind to an oncoprotein named SRSF3. How will you visualize the protein-drug interaction and gain more idea about the binding site and its interacting amino acids? You will perform a protein purification followed by LC-MS Perform a drug toxicity assay such as MTT along with the drug Search Protein Data Base (PDB) for its structure and perform BLAST to know drug binding sites Perform a molecular docking followed by visualization using bioinformatics tools such as PyMol

Distribution: Tissue Binding and StorageE.g. Usually with albumin a plasma protein and sometimes with fat tissueoOnly free or unbound drug molecules can act on a receptoro‘bound’ drug is unable to bind with a cell receptor so cannot actHow is drug binding and storage significant in drug therapy?

An allosteric interaction between a ligand and a protein is one in which:Answers: A. two different ligands can bind to the same binding site.B. the binding of the ligand to the protein is covalentC. multiple molecules of the same ligand can bind to the same binding siteD. the binding of a molecule to a binding site affects the binding of an additional molecule to the same siteE. the binding of a molecule to a binding site affects the binding properties of another site on the protein.

a drug that binds to non active site of receptor and decreases constitutive activity of receptor is known as