Context: Turn the following into a 100 word hypothesis: It is hypothesized that if water samples are collected from sites further away (downstream) from the Eastern Sewage Treatment Plant, then those samples will exhibit higher levels of contamination and thus concentration of faecal coliforms. High levels of precipitation across the South-East of Melbourne could have led to sewage spilling into the adjoining Patterson River and as a result the flow of the river may have carried these contaminants downstream towards the Mouth into Port Phillip Bay. As measured by the ONPG test, if the distance from the sewage plant of where the sample was taken increases, then these samples will exhibit an increase in their absorbance values due to the greater amount of β-galactosidase present and therefore concentration of faecal coliforms. Similarly, in terms of the MPN test, the further away from the treatment plant, the greater the number of pink colonies formed and therefore the concentration of gram-negative lactose fermenting bacteria.
Question
Context: Turn the following into a 100 word hypothesis: It is hypothesized that if water samples are collected from sites further away (downstream) from the Eastern Sewage Treatment Plant, then those samples will exhibit higher levels of contamination and thus concentration of faecal coliforms. High levels of precipitation across the South-East of Melbourne could have led to sewage spilling into the adjoining Patterson River and as a result the flow of the river may have carried these contaminants downstream towards the Mouth into Port Phillip Bay. As measured by the ONPG test, if the distance from the sewage plant of where the sample was taken increases, then these samples will exhibit an increase in their absorbance values due to the greater amount of β-galactosidase present and therefore concentration of faecal coliforms. Similarly, in terms of the MPN test, the further away from the treatment plant, the greater the number of pink colonies formed and therefore the concentration of gram-negative lactose fermenting bacteria.
Solution
The hypothesis is that water samples taken further downstream from the Eastern Sewage Treatment Plant will show higher contamination levels, particularly in faecal coliform concentrations. This is due to potential sewage spillage into the Patterson River, especially during high precipitation in Melbourne's South-East, which could carry contaminants downstream towards Port Phillip Bay. The ONPG test is expected to show increased absorbance values in these samples, indicating higher β-galactosidase levels and thus, more faecal coliforms. Similarly, the MPN test is expected to show more pink colonies the further the sample is from the plant, indicating a higher concentration of gram-negative lactose fermenting bacteria.
Similar Questions
write a hypothesis clearly outlining the independent and dependent variables for the following aim of an experiment: To determine the presence of gram-negative bacteria, i.e. faecal coliforms, in water samples collected at six different sampling sites along the Patterson River, by quantifying the concentration of faecal coliforms through conducting the ONPG Test (Colilert Method) and the Most Probable Number (MPN) Test. Moreover, the attainment of these measures will be compared with permissible governmental standards, known as the EPA guidelines, and suitability for primary and secondary recreation contact recreation evaluated.
what is the effect of false-positives and false-negative results in the Colilert Method (ONPG test) and how do these affect the concentration of faecal coliforms in water samples?
True or False QuestionTrue or false: It is estimated that less than 35 percent of urban residents in developing nations have satisfactory sewage treatment.True false question.TrueFalse
What is a common test to determine fecal contamination?Group of answer choicesMicroscopic Particulate AnalysisHeterotrophic Plate CountDensity Mass Ratio
What is a common test to determine fecal contamination?Group of answer choicesHeterotrophic Plate CountMicroscopic Particulate AnalysisDensity Mass Ratio
Upgrade your grade with Knowee
Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.