SELECT ONE OR MORE experiments are clonal analyses of haematopoietic stem and progenitor cell function.injecting mouse bone marrow cells into a sub-lethally irradiated mouse and examining the appearance of the spleen 12-14 days later.culturing human bone marrow aspirate cells in semi-solid methylcellulose cultures.profiling the different types of cells in a human blood sample by FACS (fluorescent activated cell sorting) following immunostaining with antibodiesusing genetic-based colour barcoding to enumerate the number of haematopoietic stems cells engrafting after a transplantation experiment in zebrafish.
Question
SELECT ONE OR MORE experiments are clonal analyses of haematopoietic stem and progenitor cell function.injecting mouse bone marrow cells into a sub-lethally irradiated mouse and examining the appearance of the spleen 12-14 days later.culturing human bone marrow aspirate cells in semi-solid methylcellulose cultures.profiling the different types of cells in a human blood sample by FACS (fluorescent activated cell sorting) following immunostaining with antibodiesusing genetic-based colour barcoding to enumerate the number of haematopoietic stems cells engrafting after a transplantation experiment in zebrafish.
Solution
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"Injecting mouse bone marrow cells into a sub-lethally irradiated mouse and examining the appearance of the spleen 12-14 days later." - This is a clonal analysis experiment. It involves transplanting bone marrow cells, which include hematopoietic stem and progenitor cells, into a recipient mouse and then examining the resulting hematopoietic colonies in the spleen.
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"Culturing human bone marrow aspirate cells in semi-solid methylcellulose cultures." - This is also a clonal analysis experiment. The semi-solid culture medium allows individual cells to proliferate into colonies, each of which is derived from a single progenitor cell.
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"Profiling the different types of cells in a human blood sample by FACS (fluorescent activated cell sorting) following immunostaining with antibodies." - This is not a clonal analysis experiment. It is a cell profiling experiment that uses FACS to sort and identify different cell types based on their immunostaining properties.
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"Using genetic-based colour barcoding to enumerate the number of haematopoietic stems cells engrafting after a transplantation experiment in zebrafish." - This is a clonal analysis experiment. The color barcoding allows for the tracking of individual stem cells and their progeny after transplantation.
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