One of the SNPs of a certain bitter taste receptor (TAS2R38) was investigated by PCR-RFLP method using the Fnu4H I restriction endonuclease. If the C allele is present as a template, the enzyme cleaves the PCR product amplified from it at the SNP, and the PCR product always contains two additional non-polymorphic cleavage sites. In the upper, schematic part of the figure, the cleavage sites in the expected PCR product are shown by bold arrows, and the distances between them and from the ends are shown by numbers. The lower part of the figure shows the final results of a series of experiments. Select the correct statement(s).Question 2Answer1.A technical error occured during the experint, because the PCR product of 331 + 75 + 21 + 41 = 468 bp is not visible in any lane.2.The DNA molecules migrate from the loading wells towards the positive pole during the gel electrophoresis.3.The lower part of the figure shows the results of a horizontal agarose gel electrophoresis.4.In lane No. 4 a sample of a heterozygous person with CT genotype ran.

Genotyping of a taste receptor polymorphism by PCR-RFLP.Consider DNA purification and put the steps in the correct order.

Although there seems to be only one gene that controls whether or not people taste bitterness there are more than two possible alleles at this gene locus. Therefore, there are more than two different possible allelic combinations, but people with the gene present will taste bitterness to the same extent.

Genotyping of a taste receptor polymorphism by PCR-RFLP.Which of the materials listed below were necessary to prepare the PCR reaction mixture?Question 12Answer1.short, single stranded DNA molecules2.bromophenol blue3.DNA-dependent DNA polymerase4.TaqMan probe5.dATP, dCTP, dGTP and dTTP

Structurally, taste receptors are __________Blank.Multiple Choicehair cellsmodified connective tissue cellsfree nerve endingsmodified epithelial cells

Which evidence supports labeled line coding in the taste system?Group of answer choicesIncreasing concentrations of tastants can overcome a genetic mutation that results in a deficiency of taste receptors.The same behavioral responses are observed in response to all tastants when specific taste receptors are inactivated.No change in cranial nerve activity is observed when specific taste receptors are inactivated.Mice re-expressing PLCb2 in T2-expressing taste cells in a PLCb2 mutant mouse are able to respond to bitter tastes.Receptor proteins re-expressing are expressed broadly in taste cells and not in subsets