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Which of the following is not a physical method for extraction of enzymes?*a) Osmotic shockb) Ultrasonicationc) Homogenizationd) Freezing technique

Question

Which of the following is not a physical method for extraction of enzymes?*a) Osmotic shockb) Ultrasonicationc) Homogenizationd) Freezing technique

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Solution

The question is asking which of the listed methods is not a physical method for extracting enzymes.

a) Osmotic shock - This is a physical method. It involves changing the solute concentration around the cells, causing them to either take in water and burst (in a hypotonic solution) or lose water and shrink (in a hypertonic solution). This can release the enzymes contained within the cells.

b) Ultrasonication - This is also a physical method. It involves using high frequency sound waves to disrupt cell membranes and release the enzymes.

c) Homogenization - This is a physical method as well. It involves mechanically breaking down the cells to release the enzymes.

d) Freezing technique - This is not typically considered a physical method of enzyme extraction. While freezing can cause cells to burst and release their enzymes, it is not usually used as a primary method of enzyme extraction. It is more often used as a way to preserve enzymes after they have been extracted by other means.

So, the answer is d) Freezing technique.

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