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What is the goal number of CFUs on a spread plate if a serial dilution is done correctly?

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What is the goal number of CFUs on a spread plate if a serial dilution is done correctly?

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Solution

The goal number of Colony Forming Units (CFUs) on a spread plate, if a serial dilution is done correctly, is typically between 30 and 300. This range is considered ideal because it is manageable to count and minimizes the chance of error. If there are too few colonies, the results may not be representative of the original sample. If there are too many, they may merge together, making it impossible to count individual colonies.

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