The host used for the preparation of Hepatitis-B vaccine through Recombinant DNA technology is Yeast (Saccharomyces cerevisiae).

Here are the steps involved:

1. The gene coding for the Hepatitis B surface antigen (HBsAg) is isolated from the Hepatitis B virus.

2. This gene is inserted into a plasmid, a small, circular piece of DNA found in bacteria and yeast.

3. The plasmid, now containing the HBsAg gene, is inserted into yeast cells.

4. The yeast cells read the genetic code in the HBsAg gene and produce the Hepatitis B surface antigen. This is the active ingredient in the vaccine.

5. The yeast cells are grown in large quantities, then harvested and broken open to collect the HBsAg.

6. The HBsAg is purified and used to produce the vaccine. When injected into the body, it stimulates the immune system to produce antibodies against Hepatitis B, providing immunity against the virus.

Question

The host used for the preparation of Hepatitis-B vaccine through Recombinant DNA technology is Yeast (Saccharomyces cerevisiae).

Here are the steps involved:

1. The gene coding for the Hepatitis B surface antigen (HBsAg) is isolated from the Hepatitis B virus.

2. This gene is inserted into a plasmid, a small, circular piece of DNA found in bacteria and yeast.

3. The plasmid, now containing the HBsAg gene, is inserted into yeast cells.

4. The yeast cells read the genetic code in the HBsAg gene and produce the Hepatitis B surface antigen. This is the active ingredient in the vaccine.

5. The yeast cells are grown in large quantities, then harvested and broken open to collect the HBsAg.

6. The HBsAg is purified and used to produce the vaccine. When injected into the body, it stimulates the immune system to produce antibodies against Hepatitis B, providing immunity against the virus.

Knowee AI · Accepted Answer

The host used for the preparation of Hepatitis-B vaccine through Recombinant DNA technology is Yeast (Saccharomyces cerevisiae).

Here are the steps involved:

1. The gene coding for the Hepatitis B surface antigen (HBsAg) is isolated from the Hepatitis B virus.

2. This gene is inserted into a plasmid, a small, circular piece of DNA found in bacteria and yeast.

3. The plasmid, now containing the HBsAg gene, is inserted into yeast cells.

4. The yeast cells read the genetic code in the HBsAg gene and produce the Hepatitis B surface antigen. This is the active ingredient in the vaccine.

5. The yeast cells are grown in large quantities, then harvested and broken open to collect the HBsAg.

6. The HBsAg is purified and used to produce the vaccine. When injected into the body, it stimulates the immune system to produce antibodies against Hepatitis B, providing immunity against the virus.

Recombinant DNA technology has allowed the production of Hepatitis-B vaccine. Which host is used for this preparation?

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