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You have a DNA sequence. You want to know which protein in the main protein database (“nr,” the nonredundant database) is most similar to some protein encoded by your DNA. Which program should you use? (a) BLASTN; (b) BLASTP; (c) BLASTX; (d) TBLASTN; or (e) TBLASTX.

Question

You have a DNA sequence. You want to know which protein in the main protein database (“nr,” the nonredundant database) is most similar to some protein encoded by your DNA. Which program should you use? (a) BLASTN; (b) BLASTP; (c) BLASTX; (d) TBLASTN; or (e) TBLASTX.

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Solution

You should use the program (c) BLASTX. This program translates the DNA sequence in all six possible reading frames and compares it to the protein database. It's the best choice when you have a DNA sequence and want to find the most similar protein in a protein database.

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