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Scientists investigated the effect of pH on the activity of the enzyme catalase in a fungus. The table below shows the scientists’ results.pH Enzyme activity in arbitrary units Test 1 Test 2 Test 3 Test 4 Test 5 Mean 3.0 0 0 0 0 0 0 4.0 6 5 8 4 7 6 5.0 38 65 41 42 39 5.5 80 86 82 84 88 84 6.0 100 99 96 103 102 100 6.5 94 92 90 93 91 92 7.0 61 63 61 62 63 62 8.0 22 22 21 24 21 22 Page 12 of 19The independent variables are the different parts of the bean seedling.The dependent variable is the amount of catalyst used The control variables are the temperature, concentration of hydrogen peroxide and the time for the investigationfirst get fresh bean seedling of the same age and sizethen get 5 test stubes labelled for each part of the bean seedling: seed, stem, root, leaf and seed coat and add the same volume of hydrogen peroxide to each test tube (25cm^3). Keep a separate 6th test tube with only hydrogen peroxide to compare against.cut a standardized piece of each part of the bean seedling seed, stem, root, leaf and seed coat and add them to their retrospective test tube.Observe and record the production of oxygen gas (which will be indicated by the amount of foam produced) after a certain time e.g. 30 seconds measure the height and volume of the foam and record it for each test tubeCompare the amount of foam produced for each test tube and that tells us the amounts of catalyst in the different parts of the bean seedling(i) Calculate the mean enzyme activity at pH 5.0. ______________________________________________________________ ______________________________________________________________ Mean = _____________ arbitrary units (2) (ii) On the graph paper in Figure 3, draw a graph to show the scientists’ results. Remember to: • add a label to the vertical axis • plot the mean values of enzyme activity • draw a line of best fit. Figure 3(4

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Scientists investigated the effect of pH on the activity of the enzyme catalase in a fungus. The table below shows the scientists’ results.pH Enzyme activity in arbitrary units Test 1 Test 2 Test 3 Test 4 Test 5 Mean 3.0 0 0 0 0 0 0 4.0 6 5 8 4 7 6 5.0 38 65 41 42 39 5.5 80 86 82 84 88 84 6.0 100 99 96 103 102 100 6.5 94 92 90 93 91 92 7.0 61 63 61 62 63 62 8.0 22 22 21 24 21 22 Page 12 of 19The independent variables are the different parts of the bean seedling.The dependent variable is the amount of catalyst used The control variables are the temperature, concentration of hydrogen peroxide and the time for the investigationfirst get fresh bean seedling of the same age and sizethen get 5 test stubes labelled for each part of the bean seedling: seed, stem, root, leaf and seed coat and add the same volume of hydrogen peroxide to each test tube (25cm^3). Keep a separate 6th test tube with only hydrogen peroxide to compare against.cut a standardized piece of each part of the bean seedling seed, stem, root, leaf and seed coat and add them to their retrospective test tube.Observe and record the production of oxygen gas (which will be indicated by the amount of foam produced) after a certain time e.g. 30 seconds measure the height and volume of the foam and record it for each test tubeCompare the amount of foam produced for each test tube and that tells us the amounts of catalyst in the different parts of the bean seedling(i) Calculate the mean enzyme activity at pH 5.0. ______________________________________________________________ ______________________________________________________________ Mean = _____________ arbitrary units (2) (ii) On the graph paper in Figure 3, draw a graph to show the scientists’ results. Remember to: • add a label to the vertical axis • plot the mean values of enzyme activity • draw a line of best fit. Figure 3(4

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How does a decrease in pH typically affect enzyme activity?AIncreases activity by adding more substratesBAlters the enzyme's shape by affecting ionic bondsCPrevents denaturation by forming new peptide bondsDFacilitates the release of the product from the enzyme

7.What happens to the enzyme's active site above the optimum pH?

A pH of 2 is optimal for specific digestive enzymes found in a pig's stomach. If these enzymes are placed into a neutral substrate solution, what do you predict will be the outcome? The enzyme reaction time would decrease. The enzyme reaction time would increase. The enzyme reaction time would stay the same. The optimal pH of the enzyme would change.

Write a note on any two factors that are known to affect the activity of enzymes.

What is the biological function of catalase in fruits?

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