Which of the following methods are used for detection of DNA and RNA,respectively?(A) Southern and Northern blotting(B) Southern and Western blotting(C) Northern and Southern blotting(D) Northern and Western blotting
Question
Which of the following methods are used for detection of DNA and RNA,respectively?(A) Southern and Northern blotting(B) Southern and Western blotting(C) Northern and Southern blotting(D) Northern and Western blotting
Solution
The correct answer is (A) Southern and Northern blotting.
Here's why:
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Southern Blotting: This method is used for detection of a specific DNA sequence in DNA samples. It was named after its inventor, Edwin Southern. The DNA detection is done by hybridization of the sample to a specific DNA probe.
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Northern Blotting: This method is used for studying gene expression by detection of RNA (or isolated mRNA) in a sample. Like Southern blotting, it involves gel electrophoresis to separate RNA samples by size and detection is done by hybridization to a labeled RNA probe. It was named 'Northern' as a play on the 'Southern' blotting technique.
Therefore, Southern blotting is used for DNA detection and Northern blotting is used for RNA detection.
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