How many example given below are the example  of in-situ conservation? Tissue culture, pollen bank, national park, Biosphere reserve, Botanical gardenA Three B Two C One D Five

To determine the number of examples that are considered in-situ conservation, we need to understand what in-situ conservation means. In-situ conservation refers to the conservation of species and ecosystems in their natural habitats.

Now, let's analyze each example given:

1. Tissue culture: Tissue culture is a technique used to grow and maintain plant cells or tissues in a laboratory setting. This method does not involve conserving species in their natural habitats, so it is not an example of in-situ conservation.

2. Pollen bank: A pollen bank is a facility that stores and preserves pollen samples from various plant species. This method does not involve conserving species in their natural habitats, so it is not an example of in-situ conservation.

3. National park: A national park is an area designated by a government to protect and preserve natural habitats and wildlife. National parks are considered examples of in-situ conservation because they aim to conserve species and ecosystems in their natural habitats.

4. Biosphere reserve: A biosphere reserve is a protected area that aims to conserve biodiversity while promoting sustainable development. Biosphere reserves are considered examples of in-situ conservation because they focus on conserving species and ecosystems in their natural habitats.

5. Botanical garden: A botanical garden is a curated collection of plants for scientific, educational, and conservation purposes. While botanical gardens play a role in ex-situ conservation (conserving species outside their natural habitats), they do not directly involve in-situ conservation.

Based on this analysis, the examples of in-situ conservation among the given options are the national park and the biosphere reserve. Therefore, the correct answer is B) Two.