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The following shows the nucleotide sequence of a small stretch of the non-coding strand of a gene. 5ʹ-AGCGGCT-3ʹ What is the corresponding sequence of the RNA generated when this stretch of the gene is transcribed? 5ʹ-AGCCGCU-3ʹ 5ʹ-UCGCCGA-3ʹ 5ʹ-GAUUAUC-3ʹ 5ʹ-UCGGCGA-3ʹ 5ʹ-AGCGGCU-3ʹ

Question

The following shows the nucleotide sequence of a small stretch of the non-coding strand of a gene. 5ʹ-AGCGGCT-3ʹ What is the corresponding sequence of the RNA generated when this stretch of the gene is transcribed? 5ʹ-AGCCGCU-3ʹ 5ʹ-UCGCCGA-3ʹ 5ʹ-GAUUAUC-3ʹ 5ʹ-UCGGCGA-3ʹ 5ʹ-AGCGGCU-3ʹ

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Solution

The corresponding sequence of the RNA generated when this stretch of the gene is transcribed would be 5ʹ-UCGCCGA-3ʹ.

Here's the step-by-step process:

  1. Transcription is the process where the DNA sequence is copied into a complementary RNA sequence.

  2. In this process, the DNA strand that serves as a template is the non-coding strand (also known as the antisense strand). The sequence given is of the non-coding strand: 5ʹ-AGCGGCT-3ʹ.

  3. The RNA polymerase reads the non-coding DNA strand from 3' to 5' and synthesizes the RNA from 5' to 3'.

  4. The RNA bases pair with the DNA bases in the following way: Adenine (A) pairs with Uracil (U), Guanine (G) pairs with Cytosine (C), Cytosine (C) pairs with Guanine (G), and Thymine (T) pairs with Adenine (A). However, in the DNA non-coding strand, Thymine (T) is replaced by Uracil (U) in the RNA.

  5. Therefore, the DNA sequence 5ʹ-AGCGGCT-3ʹ would be transcribed into the RNA sequence 5ʹ-UCGCCGA-3ʹ.

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