An ELISA test is performed to assess the amount of Adenovirus antibodies in an unknown patient serum sample.To do this the wells of an ELISA strip are coated with adenovirus antigens, a blocking step with BSA is performed and then the unknown patient serum sample is added.Following an incubation and then a wash step a secondary anti-human IgG conjugated to HRP is added. This is followed by another incubation and then a wash step, TMB substrate is added, then 5 mins later the stop solution is added and the assay is read using a spectrophotometer.This is an example of what type of ELISA?
Question
An ELISA test is performed to assess the amount of Adenovirus antibodies in an unknown patient serum sample.To do this the wells of an ELISA strip are coated with adenovirus antigens, a blocking step with BSA is performed and then the unknown patient serum sample is added.Following an incubation and then a wash step a secondary anti-human IgG conjugated to HRP is added. This is followed by another incubation and then a wash step, TMB substrate is added, then 5 mins later the stop solution is added and the assay is read using a spectrophotometer.This is an example of what type of ELISA?
Solution
This is an example of an indirect ELISA. In this type of ELISA, the test sample is added to a well coated with antigen. If the sample contains antibodies that recognize the antigen, they will bind to it. Then, a secondary antibody that recognizes human antibodies and is conjugated to an enzyme (in this case, HRP or horseradish peroxidase) is added. If the secondary antibody binds, the enzyme will convert a substrate (in this case, TMB or 3,3',5,5'-Tetramethylbenzidine) into a detectable signal. The signal is then stopped and read using a spectrophotometer.
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