DNA is loaded into a gel and an electrical current is applied. This spreads the DNA out into distinct bands. What causes the bands?Group of answer choicesDifferent sized pieces of DNA move at different speedsSome pieces of DNA can move towards the electrical chargeSome pieces have tails and swim fasterDifferent pieces of DNA have different electrical charges and are pushed harder
Question
DNA is loaded into a gel and an electrical current is applied. This spreads the DNA out into distinct bands. What causes the bands?Group of answer choicesDifferent sized pieces of DNA move at different speedsSome pieces of DNA can move towards the electrical chargeSome pieces have tails and swim fasterDifferent pieces of DNA have different electrical charges and are pushed harder
Solution
The bands in a DNA gel electrophoresis experiment are caused by different sized pieces of DNA moving at different speeds. When an electrical current is applied, the DNA fragments move towards the positive end of the gel. Smaller fragments move faster and therefore travel further than larger ones, creating distinct bands. This is because DNA has a uniform negative charge due to its phosphate backbone, so the speed at which the fragments move is not affected by their sequence or structure, but only by their size.
Similar Questions
DNA fragments separate on an electrophoresis gel with the A) largest segments traveling furthest. B) smallest segments traveling furthest. C) most negatively charged pieces traveling quickest. D) most positively charged pieces moving backward
Gel electrophoresis separates fragments of DNA according to what property? Length in nucleotides Organism they originate from Protein they code for2. QuestionWhat is the overall charge on DNA? Positive charge Negative charge DNA has no charge3. QuestionReferring to the image below, on which side of the gel tank should the positive electrode be placed so that DNA in the well migrates in the direction of the arrow? Left side Right side4. QuestionTwo fragments of DNA are loaded into a well on a gel. One fragment is 50 nucleotides in length, the other fragment is 200 nucleotides in length. A current is applied to the gel for 10 minutes. Which fragment will migrate further through the gel? The fragment that is 50 nucleotides in length The fragment that is 200 nucleotides in length Both fragments will travel the same distance5. QuestionWhat is the purpose of using a stain that binds to DNA and fluoresces under UV light? It assists with loading DNA fragments into wells It gives the DNA a negative charge so it will travel through the gel It gives the DNA a positive charge so it will travel through the gel It allows us to visualise where DNA fragments are positioned along the gel6. QuestionThe image below shows the results of a gel run. Based on these results, which of the following statements is correct? The DNA fragments in band A are shorter than the DNA fragments in band C The DNA fragments in band A are longer than the DNA fragments in band C The DNA fragments in band A are roughly the same length as the DNA fragments in band C7. QuestionBased on the gel results in the previous question, which of the following statements is correct? The DNA fragments in band B are shorter than the DNA fragments in band C The DNA fragments in band B are longer than the DNA fragments in band C The DNA fragments in band B are roughly the same length as the DNA fragments in band C8. QuestionEach person inherits two copies of a certain gene from their biological parents. There are two versions of the gene that can be inherited, version A and version B. Version A is longer than version B.DNA samples are collected from three patients. The gene of interest is copied using PCR and each sample is loaded into wells on a gel. The gel is run and a DNA profile is obtained (as seen in the image below). Which versions of the gene does each patient have? Justify your response.
There are genes on both strands of DNA along a chromosome.Group of answer choicesTrueFalseNext
During gel electrophoresis, the DNA sample is mixed with the tracking dye, in order to Choose one answer. Visualize DNA bands at the end All of the above To make the sample loading easy To reduce the carcinogenic nature of the material being used
Fill in the Blank QuestionFill in the blank question.In , the DNA consists mainly of a ring of DNA and associated proteins. In , the DNA strand is wound up in a highly tight coil called a chromosome.
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