A sample of the diluted coffee drink was run through the HPLC column under identical conditions to those used to obtain the calibration curve.The peak area obtained for this diluted sample was 2400 arbitrary units.Description of calibration curve - (x - concentration,y - peak area) - (0.010,1000), (0.020,2000), (0.030,3000), (0.040,4000), (0.050,5000),(0.060,6000)a) What is the purpose of creating a calibration curve? (1 mark)b) Determine the concentration of caffeine in g L-1, of the diluted sample of coffee drink. (1 mark)c) Determine the concentration of the caffeine in the original coffee drink in units of M. SHOW YOUR WORKING. (2 marks)d) Determine the mass of the caffeine, in grams, in 350 mL of the undiluted coffee drink. SHOW YOUR WORKING. (1 mark)

A 5.0 mL aliquot of the coffee drink containing caffeine was diluted to 50.0 mL with de-ionised water.A sample of the diluted coffee drink was run through the HPLC column under identical conditions to those used to obtain the calibration curve.The peak area obtained for this diluted sample was 2400 arbitrary units.a) What is the purpose of creating a calibration curve? (1 mark)b) Determine the concentration of caffeine in g L-1, of the diluted sample of coffee drink. (1 mark)c) Determine the concentration of the caffeine in the original coffee drink in units of M. SHOW YOUR WORKING. (2 marks)d) Determine the mass of the caffeine, in grams, in 350 mL of the undiluted coffee drink. SHOW YOUR WORKING. (1 mark)

Describe the process of creating a calibration curve in spectrophotometry (200-300 words). Address the following points: What a calibration curve is and why it is used. Steps involved in creating a calibration curve (preparing standard solutions, measuring absorbance, plotting the data). How to use a calibration curve to determine the concentration of an unknown sample. give me it with 150 words

What statements about calibration curves are true? (tick all that apply)Group of answer choicesThey can be used to determine the concentration of an unknown sample.They allow us to relate an instrument's response to an analyte.Calibration curves are only applicable to UV-Vis spectrophotometry.The can be used to determine the identity of an unknown sample.A calibration curve can be generated using a single standard sample.A calibration curve must be prepared from several standard solutions.

explain how sensors are calibrated, using different types of calibration.

5. Procedurea. Calibration curve (duplicates)1. Prepare standard solutions with the following nitrite concentrations: 0, 2.5, 5,10 µg/ml: Add the following volumes of nitrite working solution into 100 mlcalibration bottles: 0, 5, 10, 20 ml (using a 1-10 ml pipette) and complete thevolume with dH2O. (mark the bottles as: 0, 2.5µg/ml, 5µg/ml, 10µg/ml)2. Verify the calculations.3. Mix carefully.b. Protein precipitation (no replicates)1. Use an analytical balance to weigh a 10g processed meat sample into a 250mlErlenmeyer flask. Prepare a similar bottle without meat as blank.2. Add 5ml borax solution.3. Add 75ml dH2O using a plastic measuring cylinder and incubate in a boilingwater bath for 15 min. Use weights so the Erlenmeyer flasks stay in place.4. Let the Erlenmeyer flask cool down.5. Transfer the solution into a 100ml calibration bottle.6. Add 2ml Carrez II.7. Add 2ml Carrez I while mixing.8. Complete the volume with dH2O.9. Wait for precipitation to happen.10. Filtrate the supernatant (upper layer) using a No. 4 filter paper into a beaker.c. Color formation1. Use a plastic measuring cylinder to add 50ml dH2O into a 100ml calibrationbottle. Prepare technical duplicates for blanks and samples (12 bottles in total:8 for calibration curve, 2 for the sample, 2 for blank).2. For the samples and blanks: Transfer exactly 25 ml from the filtrate into thecalibration bottle using a volumetric pipette3. For the calibration curve standards: Transfer the standards into an empty vialor beaker, then transfer exactly 10 ml of the solution into a new 100 mlcalibration bottle using a 10 ml volumetric pipette*.*Note: By starting with the low concentration standard (working from low concentration tohigh) you can use the same beaker and pipette.54. Add 10 ml Sulfanilamide solution, mix and add 6ml of diluted HCl:H20 4:9solution. Wait for 5 minutes.5. Add 2 ml α-Naphthylethylenediamine solution, mix and complete the volumewith dH2O.6. Incubate in the dark for 10 minutes for color development (purple).7. Transfer 200µl of each solution into a 96-well plate, do the transfer 3 times foreach bottle in separate wells (technical triplicates).8. Read absorbance at 538nm.