elow are the absorbances of a set of β-carotene standards.Concentration (ng/ml) Absorbance0 0.000400 0.096800 0.1901000 0.2602000 0.5004000 0.990When you analysed a hexane extract of tomatoes via spectrophotometry, it recorded an absorbance of 0.700. Using the data points to calculate gradient, what would be the closest estimate for the concentration of β-carotene in the tomato sample? (Note: the pathlength was 1cm.)Selected Answer: [None Given]Answers: A. 150ng/mlB. 1500ng/mlC. 725ng/mlD. 300ng/mlE. 3000ng/m

Assume that you are asked to prepare a set of five, 1ml standards to measure β-carotene. You have been given a stock solution of 10mg/ml and you must design the concentrations of your standards to provide an appropriate range that reflects the β-carotene concentrations found in hexane extractions from various fruits. When you measure the absorbance of your five standards, your lowest concentration in the set returns an absorbance of 0.1. What would the concentration of β-carotene be in the lowest concentration standard? Round answers to 3 sf.Note: the molar absorptivity for β-carotene at 444nm in hexane is 127,500 M-1cm-1. The Mwt of β-carotene is 536.89 g/mol.Selected Answer: [None Given]Answers: A. 400ng/mlB. 421ng/mlC. 338ng/mlD. 444ng/mlE. 150ng/ml

Complete this statement:From the calibration curve of Parsol 340 in the previous question, we were able to our answer as the absorbance falls our known data points collected so we are we have the correct value.  If we tried to determine the concentration of a sample with an absorbance of 1.2, we would need to this result as the result falls our known data points collected and we would be if we have the correct value.

Suppose you measure the absorbance of a yellow dye solution in a 1.00 cm cuvette (b=1.00 cm).The absorbance of the solution at 427 nm is 0.44 . If the molar absorptivity of yellow dye at 427 nm is 27400 M–1cm–1, what is the concentration of the solution in M?

Suppose you measure the absorbance of a yellow dye solution in a 1.00 cm cuvette (b=1.00 cm).The absorbance of the solution at 427 nm is 0.53 . If the molar absorptivity of yellow dye at 427 nm is 27400 M–1cm–1, what is the concentration of the solution in M? Type answer:

Use Beer-Lamberts' Law to calculate (to three decimal places) the expected absorbance at 620nm of a 15uM solution of Bromocresol Green. The extinction co-efficient for BCG is 41500M-1cm-1 and the cell path length is 1cm.