In which of the following situations might it be desirable to produce an enzyme in laboratory strain bacteria, rather than the original host organism1 pointWhen the original host is aplant with slow growth rateWhen the original host onlyproduces low levels of the enzymeWhen the enzyme needs to betagged and purified for studyAll of the aboveNone of the above
Question
In which of the following situations might it be desirable to produce an enzyme in laboratory strain bacteria, rather than the original host organism1 pointWhen the original host is aplant with slow growth rateWhen the original host onlyproduces low levels of the enzymeWhen the enzyme needs to betagged and purified for studyAll of the aboveNone of the above
Solution
The answer is "All of the above".
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When the original host is a plant with a slow growth rate: Bacteria grow much faster than plants, so using bacteria can speed up the production process.
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When the original host only produces low levels of the enzyme: Bacteria can be genetically engineered to produce large amounts of the desired enzyme.
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When the enzyme needs to be tagged and purified for study: It's easier to manipulate bacteria to produce a tagged version of the enzyme, and bacterial cultures are easier to purify than plant tissues.
Similar Questions
Enzyme A catalyzes the conversion of substrate S to product P. When enzyme A is phosphorylated, its kcat value changes from 10 s−1 to 5 s−1 and its KM value changes from 100 μM to 20 μM. If equal concentrations of each enzyme form are used then compared to the unphosphorylated enzyme A, the phosphorylated form:A.has a higher catalytic efficiency and yields a faster reaction rate when [S] < 20 μM.B.has a higher turnover number and yields a faster reaction rate under saturating conditions.C.has a lower affinity for S and yields a slower reaction rate at all [S].D.is allosterically inhibited and yields a lower reaction rate at all [S].
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