You are a laboratory scientist and you are participating in a GMO research project. For you to start the methodology of your research you need to recall the process of Recombinant DNA Technology. Sequence the steps below. I. Insert the recombinant gene in the target organism. II. Selecting the gene of interest and a vector.III. Attach the desired gene fragment to the vector by using DNA ligase. IV. Make copies of the desired gene. V. Use restriction enzymes to remove the desired gene from the rest of the genome. Group of answer choicesIII, II, IV, V, III, III, V, I, IVII, V, IV, III, IIII, II, V, IV, I

The first step in GM process is identify the gene of interest to be transferred. Group of answer choicesTrueFalse

The first step in creating a transgenic organism is toMultiple choice question.insert recombinant DNA into recipient cells.synthesize or extract source DNA.isolate protein produced from recombinant DNA.cut a plasmid with restriction enzymes.

Which of the following technique(s) is/are involved in recombinant DNA technology? Cleavage of DNA at specific sites by restriction nucleases DNA ligation DNA cloning Nucleic acid hybridization All of the above

Which of the following procedures is different from a usual step in a recombinant DNA experiment?Group of answer choicesrecombining pieces of DNA from different organismsinducing a mutation in a source chromosomerecombining pieces of DNA from different organismscutting DNA with a restriction enzyme

What is the first step in making a transgenic organism?A.Select two parents that have desirable genetic traits and allow them to reproduce.B.Find a gene in one species that would be useful if added to the DNA of another species.C.Allow an organism with a desirable genetic trait that is recessive to reproduce asexually.D.Insert all of the DNA from the body cell of one individual into the egg cell of another.