The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics: Velocity Substrate Added (mM)1.2 51.6 102.8 202.9 403.1 100The Km for this enzyme is approximately:
Question
The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics: Velocity Substrate Added (mM)1.2 51.6 102.8 202.9 403.1 100The Km for this enzyme is approximately:
Solution
To determine the Km (Michaelis constant) for an enzyme, we need to plot the data given and find the substrate concentration at half the maximum velocity (Vmax). However, the data provided does not directly give us the Vmax. We can estimate it by observing the trend in the data.
The velocity seems to be approaching a maximum value around 3.1 as the substrate concentration increases. This suggests that the Vmax is around 3.1.
Next, we need to find the substrate concentration at half of this Vmax (which is 1.55). Looking at the data, this appears to occur between the substrate concentrations of 10 mM and 20 mM.
To get a more accurate value, we would need to interpolate between these two points. However, based on the data given, we can estimate that the Km for this enzyme is approximately 15 mM.
Please note that this is a rough estimation. For a more accurate determination of Km, more data points and a proper Michaelis-Menten plot or Lineweaver-Burk plot would be needed.
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