Given below are the steps to be followed for performing starch test on a green leaf(i) Boil the leaf in alcohol(ii) Boil the leaf in water(iii) Dip the leaf in iodine solution(iv) Wash the leaf in waterThe correct sequences of steps are(i), (iv), (ii), (iii)(ii), (iv), (i), (iii)(ii), (i), (iv), (iii)(iv), (i), (ii), (iii)
Question
Given below are the steps to be followed for performing starch test on a green leaf(i) Boil the leaf in alcohol(ii) Boil the leaf in water(iii) Dip the leaf in iodine solution(iv) Wash the leaf in waterThe correct sequences of steps are(i), (iv), (ii), (iii)(ii), (iv), (i), (iii)(ii), (i), (iv), (iii)(iv), (i), (ii), (iii)
Solution
The correct sequence of steps for performing a starch test on a green leaf is (ii), (i), (iv), (iii).
Here's why:
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Boil the leaf in water (ii): This is done first to kill the leaf. This stops any further chemical reactions from happening within the leaf.
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Boil the leaf in alcohol (i): This is done to remove the chlorophyll (green pigment) from the leaf. The alcohol breaks down the chlorophyll and the green color is removed. This step is necessary because the green color could interfere with the color change that indicates the presence of starch.
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Wash the leaf in water (iv): This is done to remove any excess alcohol from the leaf. The alcohol could interfere with the iodine solution in the next step.
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Dip the leaf in iodine solution (iii): This is the actual test for starch. If starch is present, the iodine solution will change color from brown to blue-black.
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