The lac operon is expressed when
Question
The lac operon is expressed when
Solution
The lac operon is expressed when the lactose is present and glucose is absent.
Here are the steps:
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In the presence of lactose: Lactose binds to the lac repressor protein and changes its shape. This prevents the lac repressor from binding to the operator region of the lac operon. As a result, RNA polymerase can bind to the promoter region and initiate the transcription of the lac operon genes, leading to the production of enzymes that can metabolize lactose.
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In the absence of glucose: When glucose levels are low, the cAMP levels in the cell are high. cAMP binds to the catabolite activator protein (CAP), and the cAMP-CAP complex binds to the promoter region of the lac operon. This enhances the binding of RNA polymerase to the promoter and increases the rate of transcription of the lac operon genes.
So, the lac operon is expressed when lactose is present (which inactivates the repressor) and glucose is absent (which allows the cAMP-CAP complex to enhance transcription).
Similar Questions
The inducer of the lac operon is lactose.
8. The lac operon, when using lacl, is regulated by a(n)a) activator and inducerc) activator and co-repressorb) repressor and inducerd) repressor and co-repressor
The lac operon in E. coli is a coordinately controlled set of genes required for lactose metabolism. The operon and its associated regulatory sequences are shown in the following diagram.Two parallel horizontal lines are shown with a break between the first segment and the second segment. The first segment has a box labeled promoter and a box labeled lacI followed by an unlabeled black box. The second segment begins with an unlabeled black box, followed by a box labeled CAP site, a box labeled promoter, a box labeled operator and then 3 boxes with a bracket above them with the caption structural genes. The 3 boxes are labeled lacZ, lacY and lacA.The function of each locus shown in the diagram is listed in the table below.Part FunctionPromoter Binding site for RNA polymeraselacI Gene encoding the lac repressor proteinCAP site Binding site for the catabolite activator proteinOperator Binding site for the lac repressor proteinlacZ Gene encoding the beta-galactosidase proteinlacY Gene encoding the lactose permease proteinlacA Gene encoding the galactoside transacetylase proteinThe activity of the lac operon is influenced by the availability of lactose and glucose in the cellular environment. In the presence of lactose, the lac repressor protein is released from the operator, which allows the structural genes to be transcribed at a low level. If glucose (the preferred carbon source) is absent during this time, the catabolite activator protein binds to the CAP site, causing the structural genes to be transcribed at a higher level.Which of the following is the most likely result of a mutation in the lac repressor protein that prevents binding to the operator?Choose 1 answer:Choose 1 answer:(Choice A, Checked) The catabolite activator protein will not bind to the CAP site, even in the absence of glucose.AThe catabolite activator protein will not bind to the CAP site, even in the absence of glucose.(Choice B) The lacI gene will be highly expressed, even in the presence of glucose.BThe lacI gene will be highly expressed, even in the presence of glucose.(Choice C) The structural genes will not be transcribed, even in the presence of lactose.CThe structural genes will not be transcribed, even in the presence of lactose.(Choice D) The lactose permease protein will be produced, even in the absence of lactose.DThe lactose permease protein will be produced, even in the absence of lactose.
Lactose or its analogues act as positive regulators of Lac operon by:Select one:a. Binding to repressor subunits so that the repressor cannot attached to operator locus.b. Attaching to I gene and preventing its expressionc. Attaching to promoter region and facilitating the biding of RNA polymerased. Increasing the synthesis of catabolic gene activator protein.Clear my choice
Which of the following is the inducer of the lac operon in cells? Group of answer choiceslactose allolactosegalactoseglucoseisopropylthiogalactopyranoside
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