In an E. coli transformation experiment, 0.001 µg of DNA was used to transform 100 µl of competent cells. The cells were recovered by adding 900 µl of LB media resulting in a final volume to 1000 µl. From the 1 ml of total reaction volume, 50 µl was plated and subsequently 500 colonies were obtained. The transformation efficiency for the batch of competent cells is

What is the goal of the bacterial transformation procedure? To provide a transformative laboratory experience To incorporate a plasmid and genetically transformed the cells To introduce a genomic deletion in the cells To inhibit DNA replication To induce duplication of the bacterial genome

What method was used for transformation of bacteria in the laboratory? Electroporation Enzymatic restriction digestion DNA elongation Heat shock of chemical competent cells Reverse transcription

Which bacterial cells can be transformed in a laboratory? / Watter selle kan in 'n laboratorium getransformeer word? A. Competent cells / Kompetente selle B. Endospores / Endosporee C. E. coli D. Any bacterial cells / Enige bakteriese selle

DNA can be introduced into bacterial cells by a. transformation b. transduction c. conjugation d. all of the above

How are you going to determine if the bacterial cells were transformed? / Hoe gaan u bepaal of die bakteriese selle getransformeer het? A. Treat cells with plasmid / Behandel selle met plasmied B. Treat cells with calsiumchloride / Behandel selle met kalsiumchloried C. Culture on selective plates with ampicillin / Kweek op selektiewe plate met ampisilien D. Culture on Nutrient agar / Kweek op voedings agar