What is the meaning of a quality score code of "E" at a position in a nucleotide sequence?Group of answer choicesThere is a low probability that the stated nucleotide is correct.A probability of ~1 in 4000 that the stated nucleotide is incorrect.An "E"xtreme probability that the stated nucleotide is incorrect.A probability of ~1 in 3.6 that the stated nucleotide is incorrect.A probability of ~1 in 69 that the stated nucleotide is incorrect.
Question
What is the meaning of a quality score code of "E" at a position in a nucleotide sequence?Group of answer choicesThere is a low probability that the stated nucleotide is correct.A probability of ~1 in 4000 that the stated nucleotide is incorrect.An "E"xtreme probability that the stated nucleotide is incorrect.A probability of ~1 in 3.6 that the stated nucleotide is incorrect.A probability of ~1 in 69 that the stated nucleotide is incorrect.
Solution
The quality score of a nucleotide sequence is a measure that reflects the confidence in the calling of the nucleotides (A, T, C, G) in a DNA sequence. It is often represented as a single ASCII character in a FASTQ file, which is a common format for storing sequencing data.
The quality score is encoded using the ASCII character set. The character "E" corresponds to a Phred quality score of 38 when using the ASCII 33 offset (commonly used in Illumina 1.8+ and Sanger sequencing).
The Phred quality score (Q) is logarithmically related to the error probability (P) as follows:
Q = -10 * log10(P)
So, if we have a quality score of 38:
38 = -10 * log10(P)
We can solve for P:
P = 10^(-38/10) = 0.000158
This means that there is a 0.000158 probability that the stated nucleotide is incorrect, or roughly 1 in 6331.
So, the correct answer is: There is a low probability that the stated nucleotide is correct.
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