Plot graph of the provide results and calculate the concentration and yield of bovine IgG in peak fraction.

A 20 mL protein fraction recovered from a column chromatography was analyzed for protein usingthe BCA method. The following data were the means of a duplicate analysis using BSA as astandard:2The average absorbance of a 1 mL sample was 0.44. Calculate protein concentration (mg/mL) andtotal protein quantity of this column fraction. (Hint: use Excel to establish the calibration curve)

A student prepared a cereal sample solution using a procedure similar to that in Lab 5. The original sample solution was diluted by a factor of two, and then the absorbance of the diluted sample solution was measured and recorded as (4.0x10^-1). Use the following calibration plot and the dilution factor to determine the concentration of iron, in unit of M, in the original cereal sample solution. The trendline (linear regression) as displayed on the plot is y = 11898x - 0.0112.

Describe how a graph of enzyme activity (y-axis) against enzyme concentration (x-axis) would look like.Question 2Select one:a.line with upward slopeb.line with an upward slope then a long flat topc.line with downward sloped.line with an upward slope then a downward slope

You have a solution containing 10mg/ml of protein bovine serum albumin (BSA). MW of BSA is 68000. Calculate the concentration of this solution expressed as mM (Express your answer to two decimal places, don't forget the unit symbol, do not place a space between the numerical value and the unit symbol in your answer).

Describe how a graph of enzyme activity (y-axis) against enzyme concentration (x-axis) would look like.Question 2Select one:a.line with upward slopeb.line with an upward slope then a long flat topc.line with downward sloped.line with an upward slope then a downward slopeClear my choice