WHICH OF THESE ENZYMES IS DESCRIBED HERE: These enzymes are used to recognize rotationally symmetrical sequence which is often referred as palindromic sequence. They require only Mg2+ as a cofactor and ATP is not needed for their activity, they are are widely used for mapping and reconstructing DNA in vitro because they recognize specific sites and cleave just at these sites Select one: a. Type IV restriction enzymes b. Type II restriction enzymes c. Type III restriction enzymes d. Type I restriction enzymes

The size of the DNA region specifically recognized by type II restriction enzymes is typically

Which of the following restriction enzymes would recognize and cut the following DNA sequence?-TGGATCCA--ACCTAGGT-ANSWEREcoRIAATTTTAAHindIIIAGCTTCGABamHIGATCCTAGSmaICCCGGGGGGCCC

In genetic engineering, restriction enzymes cleave the DNA at a specific site known as _____a) restrictionb) recognitionc) promoterd) sense

The recognition sequences of four Type-II restriction enzymes (RE) are givenbelow. The symbol (↓) indicates the cleavage site. Identify the RE that generatessticky ends.(A) RE1 - 5̍ G↓GATCC 3̍(B) RE2 - 5̍ CTG↓CAG 3̍(C) RE3 - 5̍ CCC↓GGG 3̍(D) RE4 - 5̍ AG↓CT 3̍

_________cleave the polynucleotide chain within, or near that sequence to give rise to discrete DNA fragments of defined length and sequences Select one: a. Type II restriction enzymes b. Type III restriction enzymes c. Type IV restriction enzymes d. Type I restriction enzymes