The process described in the question is known as Southern blotting, a method used in molecular biology for detection of a specific DNA sequence in DNA samples. The correct answer is B. Autoradiography.

Here are the steps:

1. The DNA sample is cut into fragments by restriction enzymes and then separated by gel electrophoresis.

2. The separated DNA fragments are then transferred to a membrane through a process called blotting.

3. The membrane is then exposed to a probe - a single DNA sequence tagged with a radioactive molecule. This probe will hybridize, or bind, to its complementary DNA sequence in the sample.

4. The membrane is then exposed to a photographic film. The radioactive probes will expose the film, creating dark spots that correspond to the DNA fragments they've bound to.

5. The film is then developed, and the resulting autoradiograph can be used to see where the probe bound to the DNA sample. This indicates the presence and location of the specific DNA sequence the probe was designed to detect.

So, the correct answer is B. Autoradiography, which is the process of recording the position of radioactive probes bound to the DNA on a photographic film.

Question

The process described in the question is known as Southern blotting, a method used in molecular biology for detection of a specific DNA sequence in DNA samples. The correct answer is B. Autoradiography.

Here are the steps:

1. The DNA sample is cut into fragments by restriction enzymes and then separated by gel electrophoresis.

2. The separated DNA fragments are then transferred to a membrane through a process called blotting.

3. The membrane is then exposed to a probe - a single DNA sequence tagged with a radioactive molecule. This probe will hybridize, or bind, to its complementary DNA sequence in the sample.

4. The membrane is then exposed to a photographic film. The radioactive probes will expose the film, creating dark spots that correspond to the DNA fragments they've bound to.

5. The film is then developed, and the resulting autoradiograph can be used to see where the probe bound to the DNA sample. This indicates the presence and location of the specific DNA sequence the probe was designed to detect.

So, the correct answer is B. Autoradiography, which is the process of recording the position of radioactive probes bound to the DNA on a photographic film.

Knowee AI · Accepted Answer

The process described in the question is known as Southern blotting, a method used in molecular biology for detection of a specific DNA sequence in DNA samples. The correct answer is B. Autoradiography.

Here are the steps:

1. The DNA sample is cut into fragments by restriction enzymes and then separated by gel electrophoresis.

2. The separated DNA fragments are then transferred to a membrane through a process called blotting.

3. The membrane is then exposed to a probe - a single DNA sequence tagged with a radioactive molecule. This probe will hybridize, or bind, to its complementary DNA sequence in the sample.

4. The membrane is then exposed to a photographic film. The radioactive probes will expose the film, creating dark spots that correspond to the DNA fragments they've bound to.

5. The film is then developed, and the resulting autoradiograph can be used to see where the probe bound to the DNA sample. This indicates the presence and location of the specific DNA sequence the probe was designed to detect.

So, the correct answer is B. Autoradiography, which is the process of recording the position of radioactive probes bound to the DNA on a photographic film.

A single stranded DNA (or) RNA, tagged with a radio active molecule (probe) is allowed to hybridize to its. Complementary DNA in a clone of cells followed by detection usingAPhotographic filmBAutoradio graphyCAmplificationDPCR

Question

Solution

Similar Questions

Upgrade your grade with Knowee