In the context of Western blot, what is the role of the primary antibody?To visualize the proteins on the membraneTo denature the proteins in the sampleTo transfer proteins from gel to membraneTo bind specifically to the target protein

When interacting with a primary antibody In a Western blot, the secondary antibody is best designed to bind to the primary antibody at the ___________.VH domainsFab regionsFc regionsVL domains

In a western blot experiment, the actin protein is probed with a mouse monoclonal primary antibody. The secondary HRP conjugated protein should be

When performing a Western blot, after running an SDS-PAGE gel, a transfer step is performed.The purpose of this transfer is to:Group of answer choicesAllow the proteins to be visualisedReduce the background ‘noise’ and reduce false positive resultsImmobilise proteins onto a nitrocellulose membraneSeparate the proteins based on their size and charge

What is the purpose of the Western blot technique?To separate proteinsTo sequence DNA fragmentsTo amplify DNA sequencesTo detect specific proteins in a sample

Explain the importance of the shape of an antibody.